HG week 11 lab

Flashcards based on lecture notes covering electrophoresis controls, PCR, VNTRs, and experimental protocols.

Electrophoresis Positive Control

Lambda DNA digested with HindIII is a commonly used DNA ladder.

Electrophoresis Negative Control

Undigested Lambda DNA.

PCR

Targeted form of DNA replication utilizing DNA, buffer, dNTPs, primers, and Taq polymerase across three temperature steps (denaturation, annealing, extension), repeated ~30 times.

VNTR

Variable Number of Tandem Repeats, generally sequences of between 10-100bp that are repeated alongside one another down the chromosome.

VNTR Variability

Mistakes in copying VNTR DNA are not efficiently fixed, leading to a highly variable number of copies, inherited 50/50, making them excellent targets for individual identification.

VNTR Amplification

Primers sit on either side of the VNTR, amplifying the entire repetitive DNA sequence. The PCR product size depends on the number of repeats between the primers.

ApoB VNTR

Has multiple alleles with over 10,000 unique combinations possible and a 15 bp repeat sequence (TTATAAAATATTTAA), resulting in a wide array of different banding patterns.

NET Buffer

Used in cheek cell DNA extraction protocol.

Saline Rinse

Used to remove food particles and contaminants before cheek cell collection.

Tween-20

Reagent used to resuspend cells.