Mcrobiology

What type of bacteria is this?

What type of bacteria is this?

Spirillum

What type of bacteria is this?

Coccus

What type of bacteria is this?

Bacillus

What type of attached cell is this?

Dipto-: Cells arranged in pairs

What type of attached cell is this?

Strepto- : cells in attached in chains

What type of attached cell is this?

Tetrads: Cells arranged in squares

What type of attached cell is this?

Random planes of clumped cells + planes

Describe the features of Gram Positive Bacteria

• Thick layer of peptidoglycan

• This holds on to the dye crystal violet

• Dye is washed out by ethanol

• They appear purple

Give an example of a gram positive

Stapyloccus

T/F: Penicillin is effective against Gram Positive Bacteria

True

Describe Gram Negative Bacteria

• They have a complex cell wall w/ a thin layer of peptidoglycan

• They also have a thick outer layer of lipopolysaccharide

• Crystal Violet is removed when rinsed with ethanol

• They appear pink

Give an example of Gram Negative Bacteria

E.Coli

T/F: Penicillin is not effective against Gram Negative Bacteria

True: Penicillin is not effective against GNB so Vanomycin is used instead

Described the process of A Gram Stain

• Begin with a heat-fixed emulsion

• Cover the smear with crystal violet stain for 1 minute

• Grasp the slide with a slide holder + rinse w/ distilled water

• Cover the smear w/ iodine stain for 1 minute

• Rinse w/ distilled water

• Decolorise with 95% ethanol until the run-off is clear

• Counterstain with safrinin

• Gentle blot dry in bibulous paper