Nonfermenters, Aeronomas, Vibrio, Campy, and H.pylori

Aeromonas hydrophilia is oxidase __________ and indole ___________

positive, positive

this bacteria produces a CagA exotoxin that causes inflammation

helicobacter pylori

campy blood agar inhibits

commensal fecal flora

Vibrio cholerae appears __________ on TCBS agar

yellow

What is a major symptom of Vibrio cholerae?

rice water stools

Vibrio parahaemolyticus causes gastroenteritis after ingesting contaminated ____________

seafood

The natural habitat of Vibrio spp. is

salt water or a mixture of salt and fresh water

Plesiomonas, Aeromonas and Vibrio all ferment ____________

glucose

What atmospheric makeup does Campylobacter require for growth

85% N₂

10% CO₂

5% O₂and is microaerophilic.

The gram stain of Campylobacter is

“gull wing”

gram negative bacilli

Infection with which organism often precedes the development of Guillain-Barre Syndrome

campylobacter

What type of infection does Campylobacter jejuni cause

gastroenteritis (bloody diarrhea)

In what body site would you find Helicobacter pylori

stomach

We presumptively diagnose Helicobacter pylori by testing for the production of what enzyme

urease

The TSI reaction for the "Non-fermenters" group is

K/K

An OF glucose test was performed. After incubation, both the closed and open tube were yellow. What does this indicate

oxidation and fermentation of glucose in the medium.

An OF glucose test was performed. After incubation, the open tube was yellow and the closed tube was green. What does this indicate

ONLY oxidation of glucose

An OF glucose test was performed. After incubation both the closed and open tube were green. What does this indicate

no utilization of glucose

After incubating an organism in nitrate broth the MLS added Nitrate A and Nitrate B reagents and noticed the broth turned red. What does this indicate

nitrate reduction

After incubating an organism in nitrate broth the MLS added Nitrate A and Nitrate B reagents and noticed the broth remained yellow-brown (no color change). What does this indicate

unable to interpret, further steps needed

When performing the nitrate reduction test, the MLS observed no color change upon the addition of Nitrate A and B. They added Zinc and the tube turned red. What does this indicate

nitrate has NOT been reduced

motile

oxidase positive

glucose oxidizers

nitrate reducers

pseudomonas spp

Pseudomonas is ___________ to polymyxin B and colistin

susceptible

Which organism causes hot tub folliculitis

pseudomonas aeruginosa

are mucoid strain of Pseudomonas aeruginosa are associated with higher virulence or alginate production

both

Oligella typically causes what type of infection

UTIs

Acinetobacter lwoffi is

asaccharolytic

nonmotile

glucose oxidizer

oxidase negative

Acinetobacter baumannii

is Achromobacter xylosoxidans a nitrate reducer

yes

Stenotrophomonas weakly oxidizes ________ but strongly oxidizes __________

glucose, maltose

Burkholderia mallei causes

glanders

Which of the listed organisms produce pyoverdine

pseudomonas putida

is Elizabethkingia meningosepticum nonmotile or motile

nonmotile

Which organism grows well at 42C

pseudomonas aeruginosa

Which of the listed organisms will not grow well on MAC

elizabethkingia meningoseptica

Burkholderia pseudomallei would most likely be isolated from what type of specimen

sputum

Which two non-fermenters listed are oxidase negative

stenotrophomonas maltophilia and acinetobacter

The non-fermenters generally

have the potential to be resistant to many antibiotics

name a fluorescent pigment

pyoverdine

Which word best describes the colony morphology of Acinetobacter on BAP

mucoid

What is the TSI reaction of Vibrio parahaemolyticus

K/A

Both Aeromonas and Stenotrophomonas maltophilia are positive for

DNase and gelitinase

oxidase positive

indole positive

reduces nitrate

positive for DNase and gelatinase

aeromonas hydrophilia

causes gastroenteritis or wound after exposure to contaminated water

aeromonas

oxidase positive

indole positive

beta hemolytic on BAP

yellow on TCBS

vibrio cholerae

O1 and O139 produces a toxin that causes hypersecretion of water and electrolytes

associated with rice water stools and severe dehydration

vibrio cholerae

oxidase psoitive

indole positive

halophilic

non-cholera vibrio spp.

halophilic

organisms thrives in high salt concentrations

V. parahaemolytics and V. vulnificus are what color on TCBS

blue green

(means they don’t ferment sucrose)

causes gastroenteritis associated with contaminated seafood or wound after exposure to contaminated (salt) water

non-cholera vibrio spp.

faint-staining “seagull wing”

needs a microaerophilic environment

oxidase positive

campylobacter jejuni

what agar is used for campylobacter jejuni

campy agar

campy agar is used to isolate from ________________ because it inhibits ____________

stool, coliforms

most common cause of bacterial diarrhea in US

associated with guillain-barre syndrome

associated with raw chicken, cross contamination during food prep, or animal handling

campylobacter jejuni

“U shaped”

rarely cultured but when it is it’s by a rapid urease (CLOtest) from a gastric biopsy or a urease breath test

helicobacter pylori

infection caused by peptic ulcers which can lead to gastric cancer

helicobacter pylori

gram-negative bacilli or coccobacilli

obligate aerobes

TSI: K/K

non-fermenters general chracteristics

ubiquitos in water, soil, and plants

may survive in disinfectants (ex: chlorhexidine)

contaminants in healthcare environments (plumbing) and equipment (ex: endoscope)

non-fermenters general chracteristics

can colonise skin and mucous membranes of healthcare workers and patients

metabolizes carbs oxidatively or use an alternative energy source (asaccharolytic)

resistant to many antibiotics

non-fermenters general chracteristics

causes UTIs, wounds, pneumonia, endocarditis, and septicemia

non-fermenters general chracteristics

most common non-fermenter isolate

pseudomonas aeruginosa

beta-hemolytic

metallic colonies on BAP

fruity odor

pigment: pyocyanin and pyoverdine

pseudomonas aeruginosa

motile

oxidase positive

reduces nitrate

glucose oxidizer

grows well at 42C

pseudomonas aeruginosa

causes otitis externa (swimmers ear), hot tub folliculitis, burn wounds, lung infection in CF patients, and healthcare associated infections

pseudomonas aeruginosa

biochemically similar to P. aeruginosa

no growth at 42C

nitrate negative

produces pyoverdine

pseudomonas fluorescenc and pseudomonas putida

biochemically similar to P. aeruginosa

doesn’t produce pyoverdine

pseudomonas stutzeri and pseumonas mendocina

pale yellow colonies on BAP

grows well at 42C

burkholderia cepacia

motile

weak oxidase positiveive

glucose oxidizer

nitrate negative

resistant to polymyxin B and colistin

burkholderia cepacia

causes healthcare associated infections

lung infection in CF patients

burkholderia cepacia

nonmotile

oxidase variable

reduces nitrate

glucose oxidizer

burkholderia mallei

no growth at 42C

resistant to polymyxin B and colistin

burkholderia mallei

causes glanders and zoonotic infections

burkholderia mallei

motile

oxidase positive

reduces nitrate

glcuose oxidizer

grows at 42C

resistant to polymyxin B and colistin

burkholderia pseudomallei

causes melioidosis (glanders like disease)

most common in southeast asia

burkholderia pseudomallei

motile

oxidase negative

weak glucose oxidizer

nitreate negative

DNase and gelitinase positive

grows at 42C

stenotrophomonas maltophilia

coccobacilli

round, mucoid growth on BAO

purpleish pigment on MAC

acinetobacter baumannii

nonmotile

oxidase negative

glucose oxidizer

grows at 42C

acinetobacter baumannii

coccobacilli

nonmotile

oxidase negative

asaccharolytic

no growth at 42C

acinetobacter lwoffi

asaccharolytic

microorganisms that aren’t able to break down carbs for energy (use nitrogenous substrates instead)

spread colony

fruity odor

alcaligenes faecalis

motile

oxidase positive

nitrate negative

glucose oxidation variable

alcaligenes faecalis

motile

oxidase positive

reduces nitrate

oxidizes glucose

achromobacter

coccobacilli

needs 48 hours to grow on MAC

oligella

oxidase positive

asaccharolytic

one species in nonmotile the other is motile

oligella

which oligella species is motile

O. ureolytica

which oligella species is nonmotile

O. urethralis

no growth on MAC

nonmotile

oxidase positive

glucose oxidizer

nitrate negative

elizabethkingia meningospetica

causes newborn meningitis, pneumonia, endocarditis, and bacteremia in immunocompromised

elizabethkingia meningospetica

what color is chryseobacterium (elizabethkingia meningosepticum)

yellow-orange

what color is sphingomonas

yellow

what color is methylobacterium

pink-coral

what color is rosesomonas

pink, mucoid

what color is shewenella

brownish

what color is chromobacterium

purple-black

carried by cold-blooded animals

plesiomonas shigelloides

oxidase positive

indole positive

nitrate positive

motile

plesiomonas shigelloides

“comma shaped” on gram stain

vibrio cholera

nonmotile nonfermenters

acinetobacter

chryseobacterium (elizabethkingia meningosepticum)

oxidase negative nonfermenters

stenotrophomonas maltophilia

acinetobacter lwoffi and baumanni

gram stain shows cocobacilli (non-fermenters)

acinetobacter

oligella