Biotech Semester 2 Review

specialty paper used in the blotting process

nitrocellulose

in the cheese lab the control trial group was given water. This is an example of a "sham" agent otherwise known as this

placebo

enzyme that attaches to the promoter prior to transcription

RNA polymerase

type of chromatography that uses the overall charge of a molecule to separate

ion exchange

device to measure 100ug of plasmid

analytic balance

a written list of hazards and interventions to chemicals can be found in this document

MSDS

word describing a substance's ability to act as either an acid or a base

amphoteric

type of chromatography that uses protein mass to separate

SEC

step in the biomanufacturing process after amplification in a bioreactor to isolate protein of interest

purification

PCR machine

thermocycler

type of tubing that can be used to separate out large protein molecules in a liquid medium

dialysis

3 nucleotide sequence representing an amino acid

codon

DNA enzyme that makes a copy of the template strand

polymerase

type of blotting for DNA

Southern

an increase in the molarity of an acid by 100 decreases the pH by this amount

two

type of chromatography using the conformation of a protein to separate

affinity

added to a protein sample prior to PAGE to make the molecules more negative and linear

SDS

agency that oversees workplace safety

OSHA

last stem in the biomanufacturing process after formulation

packaging

immuno assay that uses labeled antibodies to detect proteins

ELISA

type of bottle used to grow liquid cell cultures

roller

gel process for proteins

PAGE

MSDS color for special hazards

white

type of blotting for proteins

western

NTR segment of eukaryotic genes

intron

growing liquid culture on a manufacturing level requires one of these

bioreactor

a lab's plan for chemical safety can be found in this document

CHP

gloves, mask, shoe covers, apron,....

PPE

macromolecules: fats and oils

lipid

agency in charge of the drug approval process

FDA

in the cheese lab this variable type included the clotting agent

manipulated

type of shock that opens the membrane pores on bacteria

heat

phase of drug approval process where people with disease are tested to document efficiency

phase 2

step in the biomanufacturing process after purification that determines additives and buffering

formulation

type of media that contains antibodies

selective

phase of a chromatography where the sample and buffers are

mobile

type of cell commonly used in complex recombinant protein production

CHO

liquid growth media for e. coli

LB Broth

process of reading mRNA to make polypeptides

translation

where the plasmid is in miniprep after lysis and centrifugation

supernatant

macromolecule: arabinose, ribose, glucose, cellulose

carbohydrate

type of chromatography that separates molecules that are not attracted to water

hydrophobic

phase of PCR at 94C to separate DNA strands

Denaturation

absorption wavelength of bacteria

260nm

pipet to measure 500ul

P-1000

<7 - 0

acidic

3 nucleotide sequence on tRNA that a complimentary to the codon

anticodon

in the cheese lab this variable type included the amount of curds

responding

type of test that turns purple in the presence of a protein

Biuret

MSDS color for flammability

red

agency that oversees plant and animal safety in GMO applications

USDA

single celled prokaryote used in simple protein production

bacteria

type of blotting for RNA

Northern

phase of drug approval process where healthy people are tested for dosage and safety

phase 1

process of isolating plasmids from transformed bacteria

miniprep

type of sequence that a restriction enzyme cuts at

palindrome

device that measures absorption/ transmittance to determine concentration

spectrophotometer

pipet to measure 12ul

p-20

phase of drug approval process where animals are tested

preclinical

phase of a column where the resin beads are

stationary

colorimetric assay to determine protein concentration

Bradford

single celled eukaryote used in recombinant protein production

yeast

phase of drug approval process where a large mixed population is tested for safety and side effects

phase 3

device to measure 1.25g glucose

electronic balance

the picking up of plasmids by bacteria

transformation

added to bacteria in the log phase to make chemically competent

calcium chloride

agency that oversees environmental impact of biomanufacturing

EPA

phase of PCR at 56C to attach primers

annealing

process of reading DNA to make mRNA

transcription

substance that is added to a solution to maintain pH

buffer

MSDS color for chemical reactivity

yellow

ideal bacterial vector

plasmid

a document stating the proper use of equipment in a lab setting

GLP

DNA enzyme that forms a phosphodiester bond

ligase

process of reading mRNA to make cDNA

reverse transcription

used to isolate bacteria

petri dish

process that uses a shuttle to insert foreign DNA into a eukaryotic cell

transfection

enzyme category that Ecor1 belongs to

endonuclease

brings AAs to the ribosome

tRNA

used to heat bacteria on solid media

incubator

in the cheese lab this trial group type included the chymosin and buttermilk

experimental

type of electrophoresis used to separate DNA by kbp

horizontal

type of lamp used in UV- spectrophotometry

Deuterium

>7 - 14

basic

site of protein synthesis

ribosome

in the cheese lab this variable type included the tubes, milk, temperature

control

process of using current to make a membrane competent

electroporation

pipet to measure 50ul

p-200

MSDS color for health hazards

blue

measure of acidity

pH

phase of PCR at 72C to copy DNA strands

extension

absorption wavelength of DNA

260 nm

macromolecule: enzyme, actin, myosin, hemoglobin, antibody

protein

bacteria in the log phase that are mixed with CaCl2 are this

competent

maximum threshold for dosage as determined in preclinical testing

LD50

blocking agent used in the blotting process

dried milk

technique that stops the spread of contamination/ disease

aseptic

used to grow small attached cell cultures

t-flask

1st step in the drug approval process after R&D

discovery

IgG and IgM are examples of these proteins that are used in diagnostic studies due to their specificity for proteins

antibody