Microbiology ASCP

Cytochrome oxidase positive, DNAse negative, ornithine positive and is associated with gastroenteritis in children is

Plesiomonas shigelloides

Fusarium

Likely to cause mycotic Keratitis

Saccharomyces cerevisae

Bakers yeast

Presumptive test for ID of Lancefield group A strep is

Bacitracin susceptibility

Moraxella lacunata

Conjungtivitis

Blue color on simmons citrate

production of ammonia from ammonium phosphate

If bacteria produces tryptophanase to break down the A.A. tryptophan, which is positive for Kovacs reagent

Indole test

Klebsiella oxytoca

weak urease positive

Necator Americanus

Morganella morganii

non lactose fermenter (not pink on MacConkey

Class II or higher BSC

must be used for bioterrorism agents

alpha napthalamine

Nitrate

VP

alpha naphthol

Indole

aminobenzaldehyde

Phenylalamine deaminase

ferric chloride

Bacillus Anthracis

Ulcerating pustule, asia, animal hides

Fonsecaea pedrosoi

Chromoblastosis, H&E stained sections of tissue biopsy, Sclerotic bodies, Copper pennies,

Group A strep

Zone of inhibition around bacitracin disk

Serum medium and methylene blue stain originally described by Loeffler is helpful in

Corynebacterium diphtheriae

Mycobacterium Tuberculosis

2 weeks to grow, Rough looking

Mycobacterium Kansasii

Develop pigment when exposed to light (PHOTOCHROMIC)

Mycobacterium scrofulaceum

Develop deep yellow (orange) when incubated in the dark

Mycobacterium Fortuitum

White or gray rapid growing 3 days

Elkenella corrodens

Pitting of the agar, bleach smell,

Clostridium pefringens

Boxcar, gas gangrene, anaerobe, non spore forming

Hydrolysis of tyrosine, xanthine and casein

Nocardia

E blattae

Cockroaches

Edwarsella Tarda

Reptiles

Y. pestis

Rodents or other rats

Salmonella

Poultry

Fusobacterium nucleatum

Long slender fusiform bacteria, Strong indole positive, sputum upper respiratory infections

Plasmodium Vivax

>12 schizonts

Erysipelothrix rhusiopathiae

pipe cleaner motility/ test tube brush

Integrase

responsible for joining the reverse transcribed HIV DNA to the host cells DNA

Candida albicans

Spherical chlamydospores,

Strongyloides stercoralis

Staph Auerus

Grape like clusters, positive tube coagulase

Turbidity of a bacterial inoculum of a standard disk diffusion (Kirby-Bauer) Susceptibility test

0.5 Mcfarland standard

What distinguishes Vanco Resistant Enterococcus faecium and Enterococcus faecalis from other enterococcus species

non-motility

Yellow pigmented nocardioform bacterial species recovered from blood, non acid fast motile, and filamentous mycelium broke into pieces as it matured most likely ID is

Oerskovia turbata

Psuedomonas

TSI-alk/alk, Mot+, Ox+,

Acinetobacter baumannii

Nosocomial infections related to endotracheal tubes and trachiostomies, also causes peritonitis. Light lavender pink colonies growing on MacKonk agar, negative oxidase

Abiotrophia defectiva (Strep defectivus) and Granulicatella adiacens (S. adjacens) 2 species previously called Nutrient variant Streptococcus. What supplement must be added to recover in culture negative endocarditis

Pyridoxyl

Saccharolytic anaerobe that produces red fluoresencing colonies under a woods lamp

Prevotella bivia

Black pigment surrounded by black halo XLD,

Salmonella species

Epicoccum

M bovis Vs M tuberculosis

Growth inhibition by thiopine-2-carboxyline acid hydrazide (T2H)

Portion of tapeworm used for attachment to the host's intestines

Scolex

Alcaligenes faecalis

gray spready colonies, motile, non fermenter pertricihous flagella, Nitrate reduction, Citrate Pos

Sylvatic Plague

The bites of rate fleas

Streptococcus pyogenes

Full hemolysis, catalase neg

Each of the following can be used to separate B. Cereus from B. Anthracis except

Lecithinase production

Flat spreads gray-white colonies with outer zone of beta hemolysis, anaerobic plate, Colon cancer, Gram pos bacilli, central and subterminal spores

Clostridium septicum

Microbiota of the mouth, upper respiratory tract, grey convex translucent colonies

Veillonella parvula

Cystine tellurite blood agar

Corynebacterium diphtheria

Isospora belli

Propionibacterium acnes

Contaminant

Bacillary angiomatosis

Bartonella henselae

XLD with yellow white growth

Report as no salmonella or shigella isolated

Spot indole test on proteus

Indole pos strains tend to be PCN resistant

Actinomyces isareli

produce major quantities of succinic acid

Brucella switches from smooth to rough colonies associated with

diminished reactivity with brucella specific antibodies

Slide coagulation test is rapid identification for

Staphylococcus aureus

Blastocystis hominis

Beta hemolytic gram positive coccus from CSF of infant, Resistant to bacitracin. Which test should be performed to ID

CAMP test

Legionella recovery

Bronchial washing

Capnocytophaga

Dog bite

Leuconostoc mesenteroides

can not be treated with vancomycin

Slide coagulation test is based on detection of

Bound Coagulase

Francisella tularensis

Tiny weak staining, gram negative coccobacilli

14 day old colonies, yellow pigment after exposure to light

Mycobacterium Marinum

Cyst in the brain, broad capsule, Hyatid sand,

Cysticercosis

Candida parapsilosis

Not an appropriate isolation for Anaerobic isolation

MacConkeys agar

MRSA based upon

Simultaneous detection of the S. aureus orfX gene and several variants of the mecA gene

VLDL

Endogenous

Orgainsms should be maintained as stock culture and checked for encapsulated unknowns

B and C, KP, Strep Pyogenes

Strep moniliformis

Wild Rats

Francisella

Wild Rabbits

Brucella

Cattle

Pasturella

Cats

End product of Fermentative metabolism that characterizes enterbacteriaceae is

Various mixed acids

Difference between Salmonella enterica and shigella is that SALMONELLA

Animals serve as a reservoir of infection

a hemolytic, strep BE+/NACL-

Group D strep, non-enterococcus

A/A, Citrate +, Lysine +, Sim Neg/Neg/Neg, Urea Weakly Pos

KP

biggest challenge with francisella

Growth is slow

Scalded skin syndrome

Staphylococcus

Bordet Gengou potato infusion agar

Bordatella

Shigella

Non-motile

Other mycobacterium that accumulates niacin

Mycobacterium Simiae

Fusiform G-, Ox-, marginal finger-like projections recovered from oral cavity, periodontal disease

Capnocytophaga ochranea

Closely related C.diptheriae on tellurite

C. ulcerans

Mice bite, fever chills, blood culture

Strepto moniliformis

Separate B frag from Prevotella/Porphyromonas

Indole production

Bart hemoglobin-->beta chain tetramers in adults

Hemoglobin H disease

Classification of Enterobacterciae except

Motility via peritrichous flagellae

Proteus

UTI, G-, motile, Indole +, H2S

Not used for Urine culture

Routinely voided

E vermicularis infection occurs through

Ingesting or inhaling ova

Actinobacter differ from neisseria

Actinobactor is OX neg