Unit 7: Investigation of a Positive DAT

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19 Terms

1
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When performing a DAT, what tube must be used for collecting samples?

EDTA

2
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What are the steps of performing a DAT?

  1. polyspecific DAT - IS, RT, AHG, CC

If polyspecific DAT is +:

  1. monospecific IgG - IS, AHG, CC

  2. monospecific C3 - IS, RT, AHG

3
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Transfusion reactions would have a + DAT for (IgG/C3).

IgG

4
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All phases of AHG being reactive due to IgG (sometimes C3) is seen in:

warm autoimmune disease

5
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Cold autoimmune disease and pneumonia react with all cells at colder temperatures due to (IgG/C3).

C3

6
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A clotted tube stored at 4C will have a positive DAT due to (IgG/C3).

C3

7
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A weak and MF positive DAT is seen in:

transfusion reactions

8
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How do you identify the antibody causing a positive DAT during a transfusion reaction?

eluate (patient RBCs + chemical)

9
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Positive DATs are associated with what kind of diseases?

autoimmune diseases (systematic lupus erythematosus (SLE), infectious mononucleosis (IM), pneumonia, cold agglutinin syndrome (CAS))

10
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A positive DAT with non-reactive serum and eluate is seen in:

positive DAT due to medication

11
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What medication can mimic a warm autoantibody reaction?

methyldopa

12
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What is the purpose of performing an elution?

determine if + DAT is due to:

  1. alloantibody

  2. autoantibody

  3. drug

13
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When kind of antibodies can we perform and elution on?

IgG antibodies

14
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What is the principle of an elution?

method that removes antibodies from sensitized patient RBCs, creating an eluate that can be used for antibody identification

15
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A positive DAT due to an (autoantibodies/alloantibodies) is usually stronger and is not MF.

autoantibody

16
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What type of history would tell us that a patient does NOT have an alloantibody?

has NOT received transfusion in the past 3 months

17
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(Autoantibodies/Alloantibodies) will mask (autoantibodies/alloantibodies).

autoantibodies will mask alloantibodies

18
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How do we remove autoantibodies that are masking alloantibodies?

adsorption

19
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Mixing patient cells with their own plasma so autoantibodies are removed from their plasma, leaving only alloantibodies is known as an:

autoadsorption