Phar Bot Lab: Quiz 1 (Microscopy)

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51 Terms

1

Microscopy

The technique utilized to view samples and objects that cannot be seen with the unaided eye or not within the resolution range of the normal eye

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2

Microscope

The use of this is very vital in studying tiny objects such as plant cells

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3

Micro

It means small

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4

Scope

It means view

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5

Simple Microscope

It is a type of microscope that consists of a single lens

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6

Loupe

The term for single lens in a simple microscope

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7

Convex

The shape of the lens in a simple microscope

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8

Compound Microscope

It has two magnifying lenses and multiple objectives

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9

Eyepiece

It contains one set of lenses and rests loosely on the top of the body tube

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10

Head

The place where the eyepiece and ocular rests loosely

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11

Lenses

They are contained in the objective which are screwed into a revolving nosepiece at the lower end of the body tube

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12

Revolving Nosepiece

It puts the desired objectives to be used

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13

Objectives

The lens system nearest the specimen that magnifies the real image

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14

Scanner

The shortest objective used for scanning the view-field or searching for the structures in the specimen

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15

Low-power Objective

A short objective, but with larger lens opening

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16

High-power Objective

A longer objective, but with smaller lens

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17

Oil Immersion Objective

An objective that uses immersion oil

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18

4x

Magnification of scanner

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19

10x

Magnification of LPO

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20

40x

Magnification of HPO

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21

100x

Magnification of OIO

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22

Stage/Mechanical Stage

A flat platform that extends between the upper lens system and the lower set of devices for providing light

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23

Stage Clips

It holds the specimen slide in place

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24

Adjustment Knobs

They are used to laterally move the slide on the stage

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25

Coarse Adjustment Knob

It moves the stage over a greater distance and brings the specimen into approximate focus (for LPO and Scanner)

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26

Fine Adjustment Knob

It moves the stage more slowly for precise final focusing (for HPO and OIO)

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27

Condenser Lens

It causes the light rays to converge at the opening in the stage of the microscope

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28

Iris Diaphragm

It can be opened or closed to control the amount of light that enters the tube of the microscope

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29

Lever/Rotating Knob

It is provided on the condenser for operating the diaphragm

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30

Illuminator

The source of light in a microscope

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31

Mirror

An alternative source of light found below the condenser on microscopes without an illuminator

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32

Flat and Concave

The characteristics of a mirror in a microscope

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33

Ocular Micrometer

A glass disk that has an arbitrary scale or divisions

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34

Stage Micrometer

It is a thick glass slide used in calibrating the ocular micrometer

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35

0.01 mm

The value of small divisions in a stage micrometer

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36

0.1 mm

The value of big divisions in a stage micrometer

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37

Micron/Micrometer

The unit measurement of microscopic objects

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38

1 mm = 1000 mcm

Conversion equivalent of mm and mcm

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39

Calibration Factor

The size in mm of one small division in the ocular micrometer

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40

Magnification

The number of times the specimen has been enlarged by a microscope

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41

Total Magnification

It can be obtained by multiplying the magnification of the ocular lens by the magnification of the objective lens

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42

10x

The usual magnification of the eyepiece in a microscope

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43

Magnifying Power of the Eyepiece

MPE

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44

Magnifying Power of the Objective

MPO

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45

MPE x MPO

Formula for total magnification

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46

Dissecting Microscope

A type of microscope that has a three-dimensional imaging capacity used to visualize opaque objects and examine structures not easily mounted onto flat slides

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47

Stereo Microscope

Another term for a dissecting microscope

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48

1.6 mm

The diameter of the field view of the LPO

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49

0.4 mm

The diameter of the field view of the HPO

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50

6-20x

How many times does a dissecting microscope can magnify?

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51

Size of the illustration/Actual size of the specimen

Formula for the magnification of illustration

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