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Microscopy
The technique utilized to view samples and objects that cannot be seen with the unaided eye or not within the resolution range of the normal eye
Microscope
The use of this is very vital in studying tiny objects such as plant cells
Micro
It means small
Scope
It means view
Simple Microscope
It is a type of microscope that consists of a single lens
Loupe
The term for single lens in a simple microscope
Convex
The shape of the lens in a simple microscope
Compound Microscope
It has two magnifying lenses and multiple objectives
Eyepiece
It contains one set of lenses and rests loosely on the top of the body tube
Head
The place where the eyepiece and ocular rests loosely
Lenses
They are contained in the objective which are screwed into a revolving nosepiece at the lower end of the body tube
Revolving Nosepiece
It puts the desired objectives to be used
Objectives
The lens system nearest the specimen that magnifies the real image
Scanner
The shortest objective used for scanning the view-field or searching for the structures in the specimen
Low-power Objective
A short objective, but with larger lens opening
High-power Objective
A longer objective, but with smaller lens
Oil Immersion Objective
An objective that uses immersion oil
4x
Magnification of scanner
10x
Magnification of LPO
40x
Magnification of HPO
100x
Magnification of OIO
Stage/Mechanical Stage
A flat platform that extends between the upper lens system and the lower set of devices for providing light
Stage Clips
It holds the specimen slide in place
Adjustment Knobs
They are used to laterally move the slide on the stage
Coarse Adjustment Knob
It moves the stage over a greater distance and brings the specimen into approximate focus (for LPO and Scanner)
Fine Adjustment Knob
It moves the stage more slowly for precise final focusing (for HPO and OIO)
Condenser Lens
It causes the light rays to converge at the opening in the stage of the microscope
Iris Diaphragm
It can be opened or closed to control the amount of light that enters the tube of the microscope
Lever/Rotating Knob
It is provided on the condenser for operating the diaphragm
Illuminator
The source of light in a microscope
Mirror
An alternative source of light found below the condenser on microscopes without an illuminator
Flat and Concave
The characteristics of a mirror in a microscope
Ocular Micrometer
A glass disk that has an arbitrary scale or divisions
Stage Micrometer
It is a thick glass slide used in calibrating the ocular micrometer
0.01 mm
The value of small divisions in a stage micrometer
0.1 mm
The value of big divisions in a stage micrometer
Micron/Micrometer
The unit measurement of microscopic objects
1 mm = 1000 mcm
Conversion equivalent of mm and mcm
Calibration Factor
The size in mm of one small division in the ocular micrometer
Magnification
The number of times the specimen has been enlarged by a microscope
Total Magnification
It can be obtained by multiplying the magnification of the ocular lens by the magnification of the objective lens
10x
The usual magnification of the eyepiece in a microscope
Magnifying Power of the Eyepiece
MPE
Magnifying Power of the Objective
MPO
MPE x MPO
Formula for total magnification
Dissecting Microscope
A type of microscope that has a three-dimensional imaging capacity used to visualize opaque objects and examine structures not easily mounted onto flat slides
Stereo Microscope
Another term for a dissecting microscope
1.6 mm
The diameter of the field view of the LPO
0.4 mm
The diameter of the field view of the HPO
6-20x
How many times does a dissecting microscope can magnify?
Size of the illustration/Actual size of the specimen
Formula for the magnification of illustration
