Phar Bot Lab: Quiz 1 (Microscopy)

Microscopy

The technique utilized to view samples and objects that cannot be seen with the unaided eye or not within the resolution range of the normal eye

Microscope

The use of this is very vital in studying tiny objects such as plant cells

Micro

It means small

Scope

It means view

Simple Microscope

It is a type of microscope that consists of a single lens

Loupe

The term for single lens in a simple microscope

Convex

The shape of the lens in a simple microscope

Compound Microscope

It has two magnifying lenses and multiple objectives

Eyepiece

It contains one set of lenses and rests loosely on the top of the body tube

Head

The place where the eyepiece and ocular rests loosely

Lenses

They are contained in the objective which are screwed into a revolving nosepiece at the lower end of the body tube

Revolving Nosepiece

It puts the desired objectives to be used

Objectives

The lens system nearest the specimen that magnifies the real image

Scanner

The shortest objective used for scanning the view-field or searching for the structures in the specimen

Low-power Objective

A short objective, but with larger lens opening

High-power Objective

A longer objective, but with smaller lens

Oil Immersion Objective

An objective that uses immersion oil

4x

Magnification of scanner

10x

Magnification of LPO

40x

Magnification of HPO

100x

Magnification of OIO

Stage/Mechanical Stage

A flat platform that extends between the upper lens system and the lower set of devices for providing light

Stage Clips

It holds the specimen slide in place

Adjustment Knobs

They are used to laterally move the slide on the stage

Coarse Adjustment Knob

It moves the stage over a greater distance and brings the specimen into approximate focus (for LPO and Scanner)

Fine Adjustment Knob

It moves the stage more slowly for precise final focusing (for HPO and OIO)

Condenser Lens

It causes the light rays to converge at the opening in the stage of the microscope

Iris Diaphragm

It can be opened or closed to control the amount of light that enters the tube of the microscope

Lever/Rotating Knob

It is provided on the condenser for operating the diaphragm

Illuminator

The source of light in a microscope

Mirror

An alternative source of light found below the condenser on microscopes without an illuminator

Flat and Concave

The characteristics of a mirror in a microscope

Ocular Micrometer

A glass disk that has an arbitrary scale or divisions

Stage Micrometer

It is a thick glass slide used in calibrating the ocular micrometer

0.01 mm

The value of small divisions in a stage micrometer

0.1 mm

The value of big divisions in a stage micrometer

Micron/Micrometer

The unit measurement of microscopic objects

1 mm = 1000 mcm

Conversion equivalent of mm and mcm

Calibration Factor

The size in mm of one small division in the ocular micrometer

Magnification

The number of times the specimen has been enlarged by a microscope

Total Magnification

It can be obtained by multiplying the magnification of the ocular lens by the magnification of the objective lens

10x

The usual magnification of the eyepiece in a microscope

Magnifying Power of the Eyepiece

MPE

Magnifying Power of the Objective

MPO

MPE x MPO

Formula for total magnification

Dissecting Microscope

A type of microscope that has a three-dimensional imaging capacity used to visualize opaque objects and examine structures not easily mounted onto flat slides

Stereo Microscope

Another term for a dissecting microscope

1.6 mm

The diameter of the field view of the LPO

0.4 mm

The diameter of the field view of the HPO

6-20x

How many times does a dissecting microscope can magnify?

Size of the illustration/Actual size of the specimen

Formula for the magnification of illustration