Electron Microscopy: High-Resolution Imaging

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5 Terms

1
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Why does electron microscopy provide higher resolution than LM?

Because electrons have much shorter wavelengths than visible light.

2
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What is TEM (Transmission Electron Microscopy) used for?

Imaging thin sections of cells; reveals internal ultrastructure.

3
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How are proteins localized in TEM?

With antibodies attached to electron-dense gold particles (immuno-TEM).

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What is cryo-EM and why is it useful?

Imaging of hydrated, unstained, unfixed specimens at very low temperatures, preserving near-native structures.

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What does SEM (Scanning Electron Microscopy) show?

The surface features and 3D topography of specimens coated in metal.