Lysis Buffer Components

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Last updated 2:33 PM on 9/21/26
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5 Terms

1
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EDTA

  • Chelating agent

  • Deactivates DNAases by binding to magnesium and calcium


2
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Proteinase K (ProK)

  • protease enzyme

  • Breaks down proteins and deactivates DNAases


3
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SDS (Detergent)

Lyses open the cell membrane


4
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DTT

  • Breaks disulfide bonds in proteins

  • Used in Semen and Hair


5
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Extraction Workflow

  1. Proteinase K and EDTA

  2. SDS/Detergent

  3. DTT (if necessary)