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23 Terms

1

Gram-positive cell wall

A thick peptidoglycan layer that retains the Crystal Violet stain during Gram staining, appearing purple.

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2

Gram-negative cell wall

A thin peptidoglycan layer surrounded by an outer membrane that does not retain the Crystal Violet stain, appearing pink or red.

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3

Mycolic acid

A waxy substance found in the cell walls of Mycobacterium and Nocardia, providing resistance to desiccation and immune attacks.

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4

Atypical cell walls

Cell walls that lack peptidoglycan, characteristic of Archaebacterium.

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5

No cell wall

A characteristic of Mycoplasmas, such as Mycoplasma pneumoniae, which makes them resistant to antibiotics that target cell wall synthesis.

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6

Endospore

A dormant, tough, and non-reproductive structure produced by some bacteria to survive extreme conditions.

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7

Sporogenesis/Sporulation

The process by which a bacterial cell forms a spore, involving DNA replication and the formation of a protective coat.

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8

Germination

The process by which an endospore becomes a vegetative cell under favorable conditions.

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9

Exotoxins

Toxins released by bacteria into the surrounding environment, often composed of proteins.

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10

Endotoxins

Components of the outer membrane of Gram-negative bacteria, released upon cell lysis, typically lipopolysaccharides.

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11

Photoautotrophs

Organisms that obtain energy from sunlight and carbon from carbon dioxide.

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12

Chemoheterotrophs

Organisms that obtain energy from organic compounds and carbon from the same or different organic compounds.

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13

Facilitated diffusion

The process of passive transport of molecules across a cell membrane via specific transmembrane integral proteins.

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14

Active transport

The movement of molecules across a cell membrane against their concentration gradient, requiring energy.

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15

Mesophile

Bacteria that grow best at moderate temperatures, typically between 20-45°C.

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16

Thermophile

Bacteria that thrive at high temperatures, usually above 45°C.

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17

Psychrophile

Bacteria that prefer cold environments, typically between -5°C to 20°C.

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18

Exponential growth

Growth pattern where the population size doubles at regular intervals.

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19

OD (Optical Density)

A measure of how much light is absorbed by a suspension of cells, used to estimate cell concentration.

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20

Filtration

A method of cell counting that uses filters to separate cells from liquids.

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21

Direct Microscopic Methods

Techniques to count cells by observing them directly under a microscope.

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22

Turbidity

A measure of the cloudiness of a solution, correlating with cell concentration.

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23

Flow Cytometry

A technique that uses laser technology to count and analyze microscopic particles.

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