1. High oxygen levels; Increased aerobic respiration supported larger bodies and more active movements
2. Diversification of algae; Larger and more nutrient-rich photosynthetic organisms produced oxygen and served as a higher quality food source, making more energy and mass available for growth, reproduction, and diversification
3. Evolution of predation; Evolution of predation caused natural selection for shells, hard exoskeletons, rapid movement, etc.
4. New niches beget new niches; Once animals could move and use new resources, availability to exploit new niches created more new niches, driving speciation and ecological diversification
5. New genes, new bodies; Hox genes are important for organizing the development of body in diverse animals by signaling where cells are in he embryo. Increased number of hox genes allowed larger and more complex bodies to evolve