Strep pg 13-16 200 QS

Plastic or wire shafts

Fiber shafts recommended for collecting C. trachomatis or Ureaplasma urealyticum specimens to avoid interference

30% hydrogen peroxide

Reagent used in the Superoxol test for rapid N. gonorrhoeae identification

37°C

Standard incubation temperature for body fluids such as CSF or joint fluids prior to culturing

SPS (Sodium polyanethol sulfonate)

Component in blood culture broths to which N. gonorrhoeae is sensitive

0.025%

Maximum allowable percentage concentration of SPS in blood culture broths

Cytocentrifuge

Equipment used for direct Gram stain of body fluids to achieve the best cell preparation

tetramethyl-p-phenylenediamine dihydrochloride

Reagent chemical name used for the Oxidase test

Purple

Positive color reaction observed in the Oxidase test

Superoxol test

Rapid identification test used for N. gonorrhoeae isolated from urogenital sites

Vigorous bubbling

Positive visual indication in a Superoxol test

DNase test

Test used to differentiate Moraxella catarrhalis (+) from Neisseria gonorrhoeae (-)

Vancomycin

Antibiotic component in Thayer Martin agar that targets Gram-positive organisms

Lincomycin

Alternative antibiotic to Vancomycin in Thayer Martin agar

Colistin

Antibiotic component in Thayer Martin agar targeting Gram-negative organisms

Nystatin

Component in Thayer Martin agar that inhibits fungi and yeast

Trimethoprim lactate

Added antibiotic in Modified Thayer Martin (MTM) media that prevents the swarming of Proteus

Anisomycin

Antibiotic substituted for Nystatin in Martin Lewis media

Amphotericin B

Antibiotic substituted for Nystatin in New York City (NYC) media

Lysed horse blood

Specific blood component added to New York City media

Ureaplasma (U. urealyticum)

Genital mycoplasma genus that can be cultivated on New York City media along with M. hominis

GC-LECT

Selective gonococcal agar containing Vancomycin, Lincomycin, Colistin, Trimethoprim lactate, and Amphotericin B

Cotton or calcium alginate

Specimen swab material that interferes with HSV and C. trachomatis

Dacron or rayon fibers

Recommended alternative swab fibers to cotton and calcium alginate

Transgrow media

Transport medium containing TM with glucose, 2% agar, trimethoprim lactate, and incorporated $\text{CO}_2$ in a bottle

Cary Blair medium

General transport media option alongside Transgrow for fastidious organisms

Neisseria lactamica

Weak nasopharyngeal pathogen that is Gram-negative and ONPG positive

ONPG

Enzyme test responsible for the lactose positivity of Neisseria lactamica

Glucose, lactose, and maltose

Sugars fermented by Neisseria lactamica

Neisseria meningitidis

Organism for which Neisseria lactamica is often mistaken

Neisseria flavescens

Neisseria species whose name literally means "yellow"

22°C

Temperature at which N. flavescens grows on BAP or CAP to differentiate it from N. cinerea

Pharynx

Primary isolation site for Neisseria flavescens

Asaccharolytic

Term for organisms like N. flavescens and M. catarrhalis that do not ferment sugars

Neisseria cinerea

Neisseria species first described in 1906 that yields a negative glucose result in CTA sugars

CAP (Chocolate Agar Plate)

Medium on which colony morphology of N. cinerea resembles N. gonorrhoeae

Colistin susceptibility

Susceptibility test used to differentiate N. cinerea from N. gonorrhoeae

Z pattern

Pattern in which a swab specimen should be rolled on media for organisms like Acinetobacter or Capnocytophaga

Oxidase and catalase tests

Tests used to differentiate Acinetobacter spp., Capnocytophaga spp., and Kingella denitrificans from N. gonorrhoeae

Gram (-) diplococci

Morphology of Moraxella catarrhalis on Gram stain

Non-motile (NM)

Motility status of Moraxella catarrhalis

Non-hemolytic

Hemolytic property of Moraxella catarrhalis

Pili or fimbriae

Structures associated with Moraxella catarrhalis instead of a capsule

Moraxella catarrhalis

Third most common cause of otitis media and sinusitis in children

Beta-lactamase

Enzyme produced by M. catarrhalis causing resistance to penicillins

"Hockey puck" consistency

Descriptive consistency of Moraxella catarrhalis colonies on media

Upper Respiratory Tract

Primary isolation site of Moraxella catarrhalis

bromo-chloro-indolyl-butyrate

Substrate used in the Butyrate Disk Test

Blue

Positive color result in the Butyrate Disk Test

Moraxella catarrhalis

Only member of the family Neisseriaceae that hydrolyzes DNA

Neisseria mucosa

Neisseria species isolated from the airways of dolphins

Nasopharynx of children or young adults

Human site from which Neisseria mucosa is typically isolated

gamma-glutamylaminopeptidase

Enzyme responsible for pale yellow reactions produced by strains of N. mucosa and N. perflava

Neisseria polysaccharea

Neisseria species described in 1974 isolated from throats of healthy children

Growth on Nutrient agar

Test capability at 35°C used to separate N. polysaccharea from N. meningitidis

Glucose and maltose

Carbohydrates fermented by Neisseria polysaccharea

Dry, wrinkled (bread crumb-like), adherent

Appearance of Neisseria sicca colonies

Glucose, Maltose, and Sucrose

Sugars fermented by Neisseria sicca

Nasopharynx, saliva, sputum

Primary isolation sites for Neisseria sicca

Normal flora of upper respiratory tract

Normal habitat of Neisseria subflava

Clay-like consistency

Consistency of Neisseria elongata colonies

elongata, glycolytica, nitroreducens

Three subspecies of Neisseria elongata

Dogs

Normal oral habitat of Neisseria weaveri

Does not reduce nitrate, but reduces nitrite to gas

Reaction of Neisseria weaveri with nitrate and nitrite

Wound from dog bites

Primary isolation site for Neisseria weaveri in humans

Glucose (+) only

CTA sugar result for Neisseria gonorrhoeae

Glucose (+) and Maltose (+)

CTA sugar results for Neisseria meningitidis

Glucose (+), Maltose (+), and Lactose (+)

CTA sugar results for Neisseria lactamica

Glucose (+), Maltose (+), Sucrose (+), and Fructose (+)

CTA sugar results for Neisseria sicca

Positive (+)

Acid production from fructose by Neisseria sicca

Positive (+)

Selective medium growth result for Moraxella catarrhalis on Thayer Martin agar

Susceptible (S)

Bacitracin (Taxo A) susceptibility result for Group A Streptococcus

Resistant (R)

Bacitracin (Taxo A) susceptibility result for Group B Streptococcus

Positive (+)

PYR test result for Group A Streptococcus

Positive (+)

CAMP test result for Group B Streptococcus

Susceptible (S)

SXT-TMP susceptibility result for Group C, F, and G Streptococci

Resistant (R)

SXT-TMP susceptibility result for Group A and Group B Streptococci

Positive (+)

Hippurate hydrolysis result for Group B Streptococcus

Taxo N

Alternative name for the Oxidase Test paper disk

Weak or delayed bubbling

Result of Superoxol test on N. meningitidis and N. lactamica

Cystine Trypticase Agar (CTA)

Medium commonly used for carbohydrate utilization tests in Neisseria species

Phenol red

pH indicator incorporated into Cystine Trypticase Agar (CTA)

24-72 hours

Standard incubation time frame for reading CTA sugar reactions

Weak acid production

Limitation of CTA regarding N. gonorrhoeae

Misidentification of sucrose negative strains as N. meningitidis

Diagnostic limitation of CTA regarding N. subflava

Chromogenic Substrates

Diagnostic method detecting enzymes that hydrolyze colorless substrates into colored endproducts

Coagglutination method

Rapid test method utilizing antibody-coated particles applied to N. lactamica

Fluorescent Antibody testing

Immunologic assay that employs labeled monoclonal antibodies

Urethral discharge or urine

Preferred clinical specimens for NAAT diagnosis of N. gonorrhoeae and Chlamydia

MALDI-TOF

Mass spectrometry method listed for definitive identification of fastidious bacteria

Hybridization / NAAT

Molecular assay involving target sequence binding used for identification

Oxidase

Enzymatic activity positive in both M. catarrhalis and N. gonorrhoeae

beta-galactosidase, gamma-glutamyl aminopeptidase, prolyl-hydroxylprolyl aminopeptidase

Enzyme profile lacking in Moraxella catarrhalis

Similar colony morphology

Colonial appearance feature shared by N. gonorrhoeae and N. cinerea on CAP

Martin Lewis media

Selective medium containing modified TM components plus Anisomycin

Vancomycin, Colistin, Nystatin

Combination of antibiotics denoted by "VCN"

Vancomycin, Colistin, Nystatin, Trimethoprim lactate

Combination of antibiotics denoted by "VCN-T"

Vancomycin, Colistin, Anisomycin (or Amphotericin B), Trimethoprim

Combination of antibiotics denoted by "VCAT"

Presumptive evidence of gonorrhea

Direct microscopic examination of endocervical secretions in symptomatic women serves as what type of evidence

37°C

Temperature to which body fluids should be kept if not held at room temperature

Neisseria flavescens

Organism described as a yellow-pigmented Gram-negative coccus