Lab 2: Microscopes (microbio)

nanometer (nm)

nanometer (nm)

• 1/1,000,000,000

• 10^-9

micrometer (um)

• 1/1,000,00

• 10^-6

millimeter (mm)

• 1/1000

• 10^-3

prokaryote

• bacteria & archaea

• size: 1-5 um

• no nucleus / organelles

• 1 circular chromy

• replicates through binary fission (aka asexually)

eukaryotes

• fungi (yeast and mold)

• size 10um and 10mm

• has nucleus and organelles

• 2+ linear chromy

• replicates through asexual or sexual replication

visible light range

400mm - 750mm

shorter wavelength = ……

higher resolution

total magnification equation

object lens x ocular lens = total magnification

Oil immersion lens objective

100x

why does oil immersion help looking into the microscope

the light don’t refract as much. oil and glass have the same density

Transmission electron microscope (TEM)

• sample dies because the sample is vacuumed

• better for internal view

Scanning electron microscope (SEM)

• metal coating

• sample is also dead

• better for a surface view

*magnification

number of times the image of the object is enlarged

*resolution

ability of the microscope to provide detail and clarity

lambda

wavelength of light (measured in mm)

numerical aperture (NA)

property of objective lens to gather light

Field of view (fov)

area seen when looking through the oculars

*parfocal

phenomenon of partial focus when switching objectives

working distance

distance between objective and specimen

*inter-pupillary distance

distance between the two oculars

what did we do in this lab?

• look at pond water with the microscope,

• figure out and play around with the settings of the microscope