Decalcification

Ratio of tissue to decalcifying agent

1:20

TRUE OR FALSE: Heat hastens decalcification

TRUE

Most common routine decalcifying agent

Nitric acid

Nitric acid imparts a yellow color due to formation of _

Nitrous acid

How to remedy the yellow coloration of nitric acid

Add 5% Sodium Thiosulfate or use urea crystals

Most rapid decalcifying agent

Phloroglucin nitric acid

Decalcifying agent associated with:

• decalcifies and softens tissue at the same time

Perenyi’s fluid

Decalcifying agent associated with:

• recommended for surface decalcification of tissue blocks

HCl

Decalcifying agent associated with:

• recommended for teeth and small pieces of bone

Von ebner’s

BEST decalcifying agent; for routine decalcification of post mortem research tissues

Formic acid

Decalcifying agent associated with:

• both a fixative and decalcifying agent

Formic acid

TCA

Chromic acid/Flemming’s

Flemming’s is AKA

Chromic acid

Chromic acid is AKA

Flemming’s

Type of decalcifying method that hastens decalcification by using formic acid

Ion exchange resin

Formic acid decalcifying agent added is how many times the volume of tissue in Ion Exchange Resin

20-30x

In electrophoresis decalfication, Calcium ions move toward what charge

Cathode

TRUE OR FALSE: Xray method is the most ideal way of checking the decalfication of mercuric chloride fixed tissues

FALSE; it is the most ideal for everything else but mercuric chloride fixed tissues

Chemical method of measuring the extent of decalcification is AKA

Calcium oxalate

Tissue softener associated with:

• appears swollen and soapy

• No negative effect too detrimental

Molliflex

Addition of _ hastens decalcification when using formic acid

Citrate