Microbiology Lab Flashcards

Flashcards about proper lab procedure.

What does it mean if bacteria appear pink or reddish after staining?

They are Gram-negative bacteria.

What is Aseptic Technique ?

The practice and/or process of preventing contamination.

What is Etiology?

Etiology is the study of a disease or condition.

What are micro organisms

Micro organisms are microscopic organisms like bacterium, fungi, or virus

What are the types of micro organisms

Prions, Viruses, Bacteria, Protists, Fungi, Helminths

What does it mean if bacteria appear purple after staining?

They are Gram-positive bacteria.

How was the work area sterilized, according to the animation?

By wiping down the table with a 70% ethanol solution and using a bunsen burner to sterilize the inoculation loops.

How were the inoculating loops sterilized, according to the animation?

Holding it in the flame a Bunsen burner until it glows red hot.

List the PPE (personal protective equipment) the scientist was wearing.

Hair tied back, goggles, gloves, and a lab coat.

What is an inoculation loop used for?

Collecting samples.

How do you sterilize an inoculation loop?

Hold it in the flame of a Bunsen burner until it glows red hot.

What does heat fixing do?

Prevents the bacteria from being washed away during the staining process.

List the chemicals used in order, with the time each should sit.

Crystal Violet (60 seconds), Gram's Iodine (60 seconds), 95% alcohol (30 seconds), Safranin (60 seconds).

What can be identified from the staining results?

The type of bacteria found (Gram-positive or Gram-negative).