|BIO. LAB| ALCOHOL FERMENTATION

GLYCOLYSIS

metabolic road. happens in cytoplasm of all cells. glucose break into two pyruvic acids, yielding a net of two ATP.

AEROBIC RESPIRATION

normal conditions, with lots of oxygen in there, yeast carries out this.

FERMENTATION

turns pyruvic acid molecules to molecules of ethanol, releases CO2. can’t make ATP, regenerates coenzyme NAD+ from NADH.

ALCOHOL FERMENTATION

yeast is unicellular, eukaryotic fungi in baking & brewing.

RESPIRATION

well-knowned from fermention by type of molecule that accepts electrons, removed in oxidation of glucose. final electron acceptor is a inorganic molecule.

ORGANIC MOLECULE

acetaldehyde/pyruvic acid

_ ml of each solution

20

EACH LARGE (NUMBERED)

1.0 ml.

TUBE 1

10% fructose (syrup) solution with heat

TUBE 2

10% fructose (syrup) solution: no heat/room temp

TUBE 3

1% starch solution with heat

TUBE 4

1% starch solution & diastase mixture with heat

MATERIAL 1

graduated fermenation tubes (4)

MATERIAL 2

heat lamp

MATERIAL 3

10% frutose (syrup) solution

MATERIAL 4

yeast suspension

MATERIAL 5

diastase solution

MATERIAL 6

1% starch solution

PREP. FOR FERMENTATION TUBES: (1-3) 1

pipet 3ml of the yeast suspension to each fermentation tube.

PREP. FOR FERMENTATION TUBES: (1-3) 2

fill tube with assigned substrate. fill past U-curve of the tube to the base of bulb. turn tube right side up preventing formation of air bubbles.

PREP. FOR FERMENTATION TUBES: (1-3) 3

1 & 3 tubes are placed near the heat lamp; tube 2 away from the lamp.

1. PREP. FOR FERMENTATION TUBES: 4

add 3ml of the yeast suspension to the fermentation tube

2. PREP. FOR FERMENTATION TUBES: 4

use graduated cylinder, measure 10ml of the starch solution; pour into beaker.

3. PREP. FOR FERMENTATION TUBES: 4

use graduated cylinder, measure 5ml of the prepared diastase solution. add in the beaker of starch; swirl to mix throughly.

4. PREP. FOR FERMENTATION TUBES: 4

use starch & disastase mixture; pipet into tube filling past the U-curve to base of the bulb. Carefully tube right side up preventing the formation of any air bubbles.

5. PREP. FOR FERMENTATION TUBES: 4

place lamp on tubes 1 & 3

Tube _,_, _ is

1, 3, 4 is placed under lamp, 2 is room temp.

_____ _______ ___ is produced during fermentation, collects in graduated arm of the tube, forcing liquid down away from the end of the arm.

carbon dioxide gas

Amount of ______ _______ (ml) that collects in the closed end of the tube is recroded every 10, 20, 5 minutes.

carbon dioxide

RESULTS: TUBE 1 (10% fructose syrup with heat)

1st highest

RESULTS: TUBE 2 (10% fructose syrup no heat

3rd lowest

RESULTS: TUBE 3 (1% starch with heat)

4th lowest

RESULTS: TUBE 4 (1% starch + diatase with heat)

2nd highest