Lecture 2 - Affinity Chromatography

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5 Terms

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The column contains

resin with a molecule that acts as binding site for the analyte of interest

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What happens to unbound molecules?

Unbound analytes will move through the column

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What happens to bound molecules?

The bound analyte is removed or eluted using a solution that will break the interaction between analyte and bea

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Affinity chromatography is based upon

Based on AFFINITY of an analyte to a bead

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Clinical Application

Chemistry analytes such as Hgb A1C