Electron Microscopy Safety Test guide Ch 21 of Bozzola

How many emergency exits are in the CMAS building?

4

A) Acid into Water?
Or
B) Water into Acid?

A

How many fume hoods are in the CMAS Building?

12

How many student accessible fume hoods are in the CMAS building?

11

How many fire extinguishers are in the CMAS building?

4

How do you clean up OsO4 spills?

Twice the 2% volume of OsO4 with Corn Oil then covered with cat litter.

T or F
Falls are the number 1 most preventable injury in the lab.

F They are the second, cuts and burns are the first.

Is it okay to wear sunglasses when looking at the Vacuum evaporator in use?

No. Welder's glasses or fogged photographic film should be used.

T or F
Organic and inorganic acids should be stored together.

F

T or F
Nitric Acid should be stored away from other chemicals.

T

T or F
Acids should be stored on freestanding shelves.

F

T or F
After removing materials from a stock bottle, any unused chemicals may be returned back to the stock bottle after use.

F

T or F
Chemicals may be stored in a fume hood that is used for routine work.

F

T or F
Chemicals may be stored above eye level or on top shelves.

F

T or F
Toxic chemicals and/ or controlled substances must be stored in a locked area.

T

T or F
Solvents may be stored in a standard refrigerator.

F

Class A fires involve what type of combustibles?

Wood, cloth, paper, or other common combustibles.

Class A fires are best extinguished with what type of materials?

A pressurized tank usually containing plain water or wetting agents and aqueous film forming foam (AFFF).
OR a fire blanket.

Class B fires involve what type of combustibles?

Flammable liquids

Class B fires are extinguished with what type of materials?

CO2, Halon, dry chemicals such as ammonium phosphate, or AFFF.

Class C fires involve what kind of combustibles?

Live electrical cuircuts

Class C fires are extinguished by what type of materials?

CO2, Halon, Dry Chemicals.

Class D fires involve what type of combustibles?

Combustible metals like Mg, Na, or K.

Class D fires are extinguished with what type of materials?

Extinguishers with dry chemicals such as NaCl with a thermoplastic binder.

T or F
If corrosive are ingested, it is okay to neutralize the chemical with another chemical and/ or induce vomiting.

F

What are the three necessary elements to start a fire?

Fuel, Oxygen, and and Ignition source.

T or F
Cacodylate Acid is not dangerous when it comes into contact with of the skin.

F it can pass through the skin and enter the bloodstream.

T or F
It is okay to work in the lab on any new or untried procedures or protocols without notifying any laboratory supervisors.

F any new procedure or protocol MUST be verified with Lab supervisors.

T or F
Mouth pipetting is permitted in the lab.

F

T or F
Work areas must be kept clean, uncluttered, and free of physical obstruction.

T

What must be on labels on chemicals?

Chemical names of contents, date prepared, name of preparer, any special precautions, such as freezing or refrigeration, and if possible, date of expiration.

Solid chemical waste has to be disposed of in which way?

They must be placed into sealed containers, labeled with content and amount.

Liquid chemical waste has to be disposed of in which way?

They must be kept in chemically resistant containers, covered with leakproof caps, and completely labeled.

T or F
Embedding resins (in liquid form) may be dumped into the hazardous waste container.

F they must be mixed and polymerized (hardened) in an oven instead of disposed in a liquid form. container must be sealed, labeled and sent to disposal agency.

T or F
Mercury may be disposed of as elemental mercury.

F mercury must be complexed with special agents and subsequently sealed in a glass container, labeled, and sent to a disposal agency.

T or F
Soft contact lenses are an adequate form of eye protection in the lab when working in a laboratory.

F

T or F
Heavy metal salts (lead/uranium) are used to enhance the contrast of sectioned materials.

T

T or F
Less toxic chemicals should be used as substitutes whenever possible. for example, substituting phosphate instead of cacodylate buffer, or LR White instead of Spurr's embedding resin.

T

T or F
Double gloves should be worn when handling a dangerous chemical

T

Nitric Acid is incompatible with which chemicals/ materials?

ALL base metals except aluminum and special Chromium steels. It is also incompatible with hydrogen sulfide, flammable liquids, gases, copper,brass, heavy metals. It will also attack some forms of plastics, rubber, and coatings.