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overview of the light dependent reaction?
it takes place in the thylakoid membranes
here, light energy is absorbed by chlorophyll in the photosystems. the light energy excites the electrons in the chlorophyll, leading to the release from the molecule
the chlorophyll has been photoionised
some of the energy from the released electrons is used to add a phosphate group to ADP to form ATP, and some is used to reduce NADP to form reduced NADP.
ATP transfers hydrogen to the light-independent reaction
during the process, water is oxidised to water
what is the energy from the photoionisation of chlorophyll used for?
making ATP from ADP and inorganic phosphate - this is called photophosphorylation
making reduced NADP from NADP
splitting water into protons, electrons and oxygen - this is called photolysis
how are the photosystems in the thylakoid membranes linked?
by electron carriers
what are electron carriers?
proteins that transfer electrons
the photosystems and electron carriers form an electron transport chain - a chain of proteins through which excited electrons flow
what are the 4 steps in the light-dependent reaction?
light energy excites electrons in chlorophyll
photolysis of water produces protons
energy from excited electrons makes ATP
generation of reduced NADP
step 1: light energy excites electrons in chlorophyll?
light energy is absorbed by photosystem 2
the light energy excites electrons in chlorophyll
the electrons move to a higher energy level
these high energy electrons are released from the chlorophyll and move down the electron transport chain to PS1
step 2: photolysis of water?
as the excited electrons from chlorophyll leave PS2 to move down the electron transport chain, they must be replaced
light energy splits water into protons, electrons and oxygen
step 3: energy from excited electrons makes ATP?
the excited electrons lose energy as they move down the transport chain
this energy is used to transport electrons into the thylakoid, so that the thylakoid has a higher concentration of protons than the stroma- forming a protein gradient across thylakoid membrane
protons move down their concentration gradient, into the stroma, via the enzyme ATP synthase, which is embedded in the thylakoid membrane
the energy from this movement combines ADP and inorganic phosphate to form ATP
step 4: generation of reduced NADP?
light energy is absorbed by PSI which excites the electrons again to an even higher energy level
finally, the electrons are transferred to NDAP, along with a proton from the stroma, to form reduced NADP
what is the process of electrons flowing down the electron transport chain and creating a protein gradient called?
chemiosmosis
what is cyclic photophosphorylation?
it only uses PSI
it is called cyclic because the electrons from the chlorophyll molecule are not passed onto NADP, but are passed back to PSI via electron carriers
this means the electrons are recycled and can repeatedly flow though PSI
what does cyclic photophosphorylation not produce?
reduced NADP
oxygen
it only produces small amounts of ATP