Microbiology Lab Exercise 6

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35 Terms

1

Cell division where genome replication is followed directly by the physical separation into two new, identical cells

Binary Fission

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2

Cell division where genome replication is separated by a gap phase from the physical separation into two new, identical cells

Mitosis

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3

In this phase, limiting factors intensify becoming detrimental to any cell growth.

Death Phase

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4

In this phase, the cells are adjusting to the environment.

Lag Phase

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5

In this phase, the cells reach the maximum rate of cell division

Log Phase

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6

In this phase, cells will stop growing or grow very slowly.

Stationary Phase

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7

In this phase, the majority of the cells are alive, but dead cells start to accumulate.

Stationary Phase

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8

In this phase, there are many living cells and few to none dead cells.

Log Phase

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9

In this phase, there are few cells present.

Lag Phase

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10

In this phase, the dead cells take up a larger portion of the culture and little to no growth occurs.

Death Phase

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11

Of the following absorbance reading samples, which one has more cells present?

0.85

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12

Of the following absorbance reading samples, which one has fewest cells present?

0.05

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13

How many cells are present after 9 generations (starting from 1 cell) of exponential growth (2^n)

512

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14

How many cells are present after 13 generations (starting from 1 cell) of exponential growth (2^n)

8192

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15

How many cells are present after 18 generations (starting from 1 cell) of exponential growth (2^n)

262144

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16

How many cells are present after 11 generations (starting from 1 cell) of exponential growth (2^n)

2048

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17

How many cells are present after 7 generations (starting from 1 cell) of exponential growth (2^n)

128

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18

A dilution of 1 ml bacteria from broth into 1 ml broth or water

1:2

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19

A dilution of 1 ml bacteria from broth into 3 ml broth or water

1:4

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20

A dilution of 1 ml bacteria from broth into 11 ml broth or water

1:12

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21

A dilution of 1 ml bacteria from broth into 7 ml broth or water

1:8

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22

A serial dilution of 1:10 followed by 1:100 followed by 1:100 gives a final dilution of

1:100,000

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23

A serial dilution of 1:10 followed by 1:10 followed by a 1:100 gives a final dilution of

1:10,000

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24

A serial dilution of 1:10 followed by 1:10 followed by 1:10 gives a final dilution of

1:1,000

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25

A serial dilution of 1:6 followed by 1:8 gives a final dilution of

1:48

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26

A serial dilution of 1:6 followed by 1:10 gives a final dilution of

1:60

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27

A serial dilution of 1:4 followed by 1:6 gives a final dilution of

1:24

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28

A serial dilution of 1:2 followed by 1:6 gives a final dilution of

1:12

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29

A serial dilution of 1:6 followed by 1:12 gives a final dilution of

1:72

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30

A dilution of of 0.1 ml bacteria from broth in 9.9 ml broth or water

1:100

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31

A dilution of of 0.1 ml bacteria from broth in 999.9 ml broth or water

1:10,000

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32

A dilution of of 0.1 ml bacteria from broth in 99.9 ml broth or water

1:1,000

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33

A 1:1,000,000 is which dilution factor? (write the whole number, i.e. -2, -3)

-6

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34

A 1:100,000 is which dilution factor? (write the whole number, i.e. -2, -3)

-5

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35

A 1:100,000,000 is which dilution factor? (write the whole number, i.e. -2, -3)

-8

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