Lecture 1 Histotechnology
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24 Terms
What is fixation?
the stabilization of proteins to prevent autolysis and putrefaction
What is putrefaction?
destruction of tissue by bacteria
Most common fixative?
10% neutral buffered formalin
What is the ratio of fixative to tissue?
15-20 times volume
What is dehydration, clearing, and infiltration a part of?
processing
What happens right before processing?
fixation
What must be miscible with the fixative and clearing reagent?
dehydrating agent
Why must tissue be dehydrated?
paraffin wax will not infiltrate tissue cells with water
What happens if tissue is over dehydrated?
becomes hard, brittle tissue
What happens if tissue is under dehydrated?
becomes soft, mushy tissue
Most common dehydrating agent?
alcohol
What is clearing?
the removal of the dehydrating fluid from the tissue
What process makes tissue more transparent?
clearing
Which process removes the alcohol?
clearing
What must be miscible with the dehydrating agent and the infiltration medium?
clearing agent
Most common clearing agent?
xylene
What does infiltration medium do?
helps to maintain the tissue structure for cutting
Most common infiltrating medium?
paraffin
Sections are usually cut at…
4-6 microns thick
What must be removed before staining?
paraffin
Tissues “brought to water” step 1:
paraffin removed with a solvent
Tissues “brought to water” step 2:
solvent removed with alcohol
Tissues “brought to water” step 3:
alcohol removed with water
Tissues “brought to water” step 4:
slides stained