Lab Safety Flashcards

Flashcards about Fire Safety, GHS Pictograms, Laboratory Hazards, WHMIS, Biological Hazards, Chemical Hazards, Radiation, Thermal Equipment and thermometers

Fire is __.

A chemical reaction that takes place when a material reacts with oxygen.

The fire triangle consists of __.

Fuel, oxygen, and heat.

__ is the temperature at which a liquid gives off enough vapor to ignite.

The lowest temperature at which a liquid gives off enough vapor to ignite.

Class A fires can be extinguished with __.

Water, foam, multipurpose ABC dry chemical, wet chemical.

Class B fires can be extinguished with __.

Foam, multipurpose ABC dry chemical powder, carbon dioxide.

Class C fires can be extinguished with __.

Carbon dioxide, multipurpose ABC dry chemical powder.

Class D fires can be extinguished with __.

Dry chemical extinguishers designed for combustible metals.

PASS, an acronym related to fire extinguishers, stands for __.

Pull the pin, aim at the base, squeeze the lever, and sweep from side to side.

GHS Pictograms are graphic images that immediately shows the user of a hazardous product what type of is present. Some major pictograms show .

Explosion, fire, oxidizing, gas under pressure, corrosion, toxicity, health effects, less serious health effects, environment, biohazardous infectious materials.

Safety Data Sheets (SDSs) provide information about the __.

Hazards of a product and advice about safety precautions.

The supplier identified on an SDS is the initial supplier identifier which means __.

The Canadian manufacturer or the Canadian importer.

The hazards encountered in the laboratory fall into various categories such as __.

Chemical, biological, physical, psychological, and ergonomical.

To maintain air quality hazards, maintain control levels of __.

Temperature, humidity, air flow, and air quality.

Types of burns include __.

Thermal, radiation, chemical, and electrical.

For spills and splashes on the skin, __ is the first preventive measure.

Wear lab coat and gloves.

For cuts, __ is an immediate first aid measure.

Apply pressure to the wound with a sterile dressing.

The standardized emergency codes by color are essential because it gives a concise means of ensuring staff receive a common __.

COLOR, DESCRIPTION, AND USE a standard language.

__ is a comprehensive system for providing health and safety information on hazardous products intended for use, handling, or storage in Canadian workplaces.

WHMIS stands for Workplace Hazardous Materials Information System.

Workplace is regulated by certain Health and Safety legislations such as __.

Federal, provincial, municipal or laboratory guidelines.

Routine Practice used in Canada, is a combination of __.

Universal precautions and body substance isolation.

The main components of WHMIS are __.

Hazard identification, labelling, and worker education.

On a supplier label, the product identifier is __.

The brand name, chemical name, common name, generic name or trade name of the hazardous product.

On a supplier label, the signal word is __.

A word used to alert the reader to a potential hazard and to indicate the severity of the hazard.

On a supplier label, Pictograms is a __.

Hazard symbol within a red square set on one of its points.

Workplace labels must have the following information: __.

Product name, safe handling precautions, and reference to SDS.

Biological hazards are caused by __.

Animals, plants, or microbes.

The common routes of entry for biological hazards are __.

Skin or eye, inhalation, ingestion and injection.

Some examples of Biological Hazards are __.

Bacteria, fungi, yeast, molds, protozoa and viruses.

In Canada, the Ministry of Health is responsible for administration and enforcement of __.

The Human Pathogens and Toxins Act.

Containment protects __ from exposure to potentially hazardous biological material.

Personnel, the work environment, the community, and the environment.

Biological Safety Cabinets (BSC’s) are designed with having __.

Special filters known as HEPA filters.

A Class I biological safety cabinet provides __.

Personnel and environmental protection, with limited use.

A Class II biological safety cabinet provides __.

Personnel, product, and environmental protection for microbiological work.

Class III BSC’s also called glove boxes, __.

Completely protect the product, personnel, and environment.

When preparing for work in the biological safety cabinet, if the cabinet is equipped with an __, test the alarm and switch it to the “on” position.

Alarm, test the alarm and switch it to the “on” position.

A chemical is defined as a hazard if it __.

Causes harmful biological effects, is flammable, explosive or highly reactive, or has potential harmful vapors or dust.

Chemicals are classified into two major groupings of affects: __.

Health hazards and physical hazards.

Those chemicals that can cause health related effects on __ exposure, are categorized under the health hazard groupings.

Short term (acute) or long term (chronic).

Toxicity can be measured in terms of LD50 which stands for __.

Lethal dose 50 or lethal concentration 50.

LD50 is the __.

Quantity of a substance that, when administered by a particular route of entry, is expected to cause death of 50 percent of a defined animal population.

The most common route of entry for hazards for laboratory workers is via __.

Respiratory system.

Certain chemicals can cause cancer and are classified into __ groups according to The American Conference of Governmental Industrial Hygienists (ACGIH).

5.

The only confirmed A1 carcinogen associated with the laboratory is __ and hence, has been phased out of use in most labs.

Benzene.

To avoid any __, all the precautions should be properly followed.

Health hazard, all the precautions should be properly followed.

Those chemicals for which there is scientifically valid evidence that they are either __, are classified under Physical Hazard grouping.

A combustible liquid, a compressed gas, explosive, flammable, an organic peroxide, an oxidizer, pyrophoric, unstable or water-reactive.

Flammables should be stored in a grounded flammable liquid storage cabinet and __.

Separate from oxidizers.

Water reactive chemicals should be stored in __.

Cool dry location separate from aqueous solutions.

All hazardous chemicals be stored in a __.

Secure location, accessible to only authorized workers.

Flammable chemicals stored in __.

Special designated flammable storage cabinets.

Store __ by reactivity class and flammability.

Acids and bases separate from each other.

Containment refers to the combination of __.

Physical design parameters and operational practices.

Containment levels are the __ to achieve biosafety for the storage and safe handling of biohazards.

Minimum physical features and operational practices.

Containment Level 1 (CL1) applies to basic laboratory that handles any __.

Biological agent unlikely to cause diseases.

Containment Level 2 (CL2) requires the use __ to protect against the risks.

Class II BSC’s and appropriate PPE (personal protective equipment).

Containment Level 3 (CL3) handles any pathogen that can cause __.

Serious human disease and may be transmitted by the airborne route.

Containment Level 4 (CL4) is the and is suitable for facilities manipulating agents any pathogen that usually produce very serious human disease, .

Maximum containment, and often untreatable.

Routine Practices are the minimum infection prevention practices that apply to all __.

Patient care, regardless of suspected or confirmed infection status.

There are five components to Routine Practices: __.

Risk assessment, hand hygiene, and personal protective equipment.

Risk assessment happens __.

Before any task is performed.

Hands should be washed with __ frequently.

Soap and water.

Wet your hands with __.

Warm running water.

Wash the front and back of your hands, as well as between your fingers and under your nails for at least __.

15 seconds.

Alcohol-based hand rubs are excellent hand antiseptics, provided they contain more than __ alcohol.

60%.

PPE includes __.

Gloves, gowns, lab coats, shoe covers, goggles, glasses with side shields, masks, and resuscitation bags.

Gloves are for __ only.

Single-patient and single-procedure use only.

To prevent loss of protection gloves should be changed after __ of work.

30 minutes.

Gloves are for __ only.

Single-patient and single-procedure use only.

The order of donning PPE is __.

Gown, Mask/N95 respirator, eye protection, and gloves.

The order of removing PPE is __.

Gloves, gown, hand hygiene , eye protection and mask/N 95 Respirator.

The environmental control measures include __.

Equipment and work area cleaning, proper disposal of waste, and appropriate ventilation.

Administrative controls include __.

Employee training, supervisory competency and immunization.

Centrifugation is a process of __.

Separation of particles in a suspension based mainly on the particle size and density.

Centrifugation separates sample into __.

Pellet and supernatant.

Centrifuge increases the rate of sedimentation by rapidly spinning, creating __.

Centrifugal force many times of that of gravity.

When blood is centrifuged, it is separated into __.

Plasma, buffy coat, and red blood cells.

The centrifuge control panel includes the power switch, __.

Timer, speed control, and brake.

Two types of rotors are __.

Horizontal or swing rotor, and fixed angle rotor.

It is very important that the tubes in the Rotors be symmetrically loaded and __.

Balanced.

Small Bench Top centrifuges uses low speed of __.

Less than 10,000 rpm.

Microcentrifuge centrifuge’s speed if normally less than __.

15,000 rpm.

Bench top Plexiglass shield from ThermoFisher is also known as Nalgene __.

Acrylic Benchtop Beta Radiation.

Liquid Expansion (Liquid In Glass) rely on the principal of __ of liquids.

Thermally expansion.

The International System of Units (SI) for radiation measurement is the __.

Absorbed dose.

To minimize the radiation exposure, use a __ for any work involving dry powders or volatile material .

Fume hood.

The CNSC is a federal regulatory agency that __

Ensure the use of nuclear substances does not pose undue risk to the public

A __ immersion thermometer has a line or ridge called an immersion ring on the stem.

Partial

Internal exposure to any __

Radioactive nuclear material can be though inhalation, ingestion, through eyes, or cuts

Radiation is the __.

Transmission of energy in the form of waves or energized particles.

There are two kinds of radiation: __.

Ionizing and non-ionizing.

Ionizing radiation has the ability to __.

Displace an electron from an atom or a molecule, thereby producing ions.

Radiation burns are burns due to prolonged exposure to __.

Ultraviolet rays of the sun or other sources of radiation.

The types of ionizing radiations are __.

Alpha particles, beta particles, gamma rays, and X-rays.

Radiation from __, is considered to be non-ionizing radiation.

Sun, light bulbs, electric power lines, radio and TV antenna, lasers etc.

Laser is a form of non-ionizing radiation, and is __,.

Monochromatic, containing one specific wavelength of light.

As a sterilizing agent, UV radiation causes __.

Modify or break in DNA which in turn causes death of the organism.

Measurement of Temperature can be done using different units like __.

Celsius, Fahrenheit and Kelvin.

Liquid Expansion thermometer’s rely on the principal of __.

Thermal expansion of liquids.

Bimetallic thermometer uses the __ which converts the temperature into the mechanical displacement.

Bimetallic strip.

Electrical thermometers rely on the principle that the electrical resistance __.

Changes with temperature.

Blood is to be stored only in a purpose built fridge between 2 – 8°C to prevent the risk of bacterial growth also known as __.

Blood bank fridge.