LS4002 - BiochemFoundations - Moles and dilutions

? = mr x mole

mass

mass = ? x mole

Mr

What mass of glucose (C6H12O6) do we need to prepare 500ml of a 4M SOLUTION?

1) how many moles of glucose do we need?

conc of solution x vol of solution = moles needed

4mol/L x 0.5L = 2 moles needed

2) what is mass of 2 moles of Glucose?

Glucose Rmm = 180

Moles needed x Rmm = mass
(Mass = mr mole)

2 x 180 = 360

Serial Dilutions

when the samples are diluted in a specific, systematic manner (1:2; 1:10; 1:100) to determine the concentration of a sample

mass = MR X ?

MOLES

mole = conc x ?

volume

mole = ? x vol

conc

? = conc x vol

MOLE

VOLUME IS ALWAYS IN

LITRES

1 litre = how many cm^3

1000

1 litre = how many dm^3

1

1 dm^3 = how many cm^3?

1000

Concentration of a serial dilution formula

M1 x V1 = M2 x V2 (= M3 x V3...)

What does m1, v1, m2 & v2 mean?

M1 = initial conc
V2 = initial volume
M2 = concentration you wish to achieve
V2 = total final volume of the liquid