BIOC 3622- Lab 4 (Protein Quantification)

What is biophotonics?

The study between the interaction of light and biological samples.

What is the main application of biophotonics?

Cancer research.

What are the 3 most common protein assays?

1. Biuret

2. Lowry

3. Bradford

What does the Bradford assay rely on?

The interaction of a large, highly conjugated organic dye.

Which of the 3 protein assays is the most sensitive?

Bradford.

What do the Biuret and Lowry assays rely on?

They rely on chelation and redox reactions to form light absorbing compounds.

Is the Biuret assay one or two steps?

One step.

Is the Lowry assay one or two steps?

Two steps.

What is the equation for the Beer-Lambert Law?

A = ecl

In the Beer-Lambert Law, what does e stand for?

The molar extinction coefficient.

In the Beer-Lambert Law, what does l stand for?

The cuvette pathlength.

Which amino acids are mainly responsible for absorbance of proteins?

1. Tryptophan

2. Tyrosine

3. Histidine

At what wavelength do the absorptions of Tryptophan, Tyrosine, and Histidine occur?

280 nm.

What is the standard curve represented by?

The relationship between absorbance (Y) and concentration (X).