PLP 266 Lab Practical

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19 Terms

1
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<p>Bipolaris Sorokiniana</p>

Bipolaris Sorokiniana

Leaf Spot

<p>Leaf Spot</p>
2
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Microscopic signs of Leaf Spot

  • Large spores (conidium)

  • y-branching

  • Crosswalls

<ul><li><p>Large spores (conidium)</p></li><li><p>y-branching </p></li><li><p>Crosswalls</p></li></ul><p></p>
3
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Dark colored spores associated with leaf spot (Bipolaris sorokiniana)

Conidia

4
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<p>Colletotrichum cerale</p>

Colletotrichum cerale

Crown Rot Anthracnose

<p>Crown Rot Anthracnose</p>
5
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Crown Rot Anthracnose (Colletotrichum cerale) microscopic signs

  • Crescent-shaped spores

  • y-branching

  • Cross walls

<ul><li><p>Crescent-shaped spores</p></li><li><p>y-branching</p></li><li><p>Cross walls</p></li></ul><p></p>
6
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Typhula incarnata

Gray Snow Mold

<p>Gray Snow Mold</p>
7
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Gray Snow Mold (Typhula incarnata) microscopic signs

  • Clamp connections

  • No spores

  • y-branching

  • Cross walls

<ul><li><p>Clamp connections</p></li><li><p>No spores</p></li><li><p>y-branching</p></li><li><p>Cross walls</p></li></ul><p></p>
8
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<p>Rhizoctonia solani</p>

Rhizoctonia solani

Brown Patch

<p>Brown Patch</p>
9
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Brown Patch (Rhizoctonia solani) microscopic signs

  • 90 degree branching

  • No spores

  • Cross walls

<ul><li><p>90 degree branching</p></li><li><p>No spores</p></li><li><p>Cross walls</p></li></ul><p></p>
10
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<p>Clarireedia jacksonii</p>

Clarireedia jacksonii

Dollar Spot

<p>Dollar Spot</p>
11
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Dollar Spot (Clarireedia jacksonii) Microscopic signs

  • y-branching

  • Cross walls

  • No spores

<ul><li><p>y-branching</p></li><li><p>Cross walls</p></li><li><p>No spores</p></li></ul><p></p>
12
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Pythium spp. Microscopic signs

  • y-branching

  • No spores

  • No cross walls

<ul><li><p>y-branching</p></li><li><p>No spores</p></li><li><p>No cross walls</p></li></ul><p></p>
13
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<p>Pythium root rot disfunction microscopic signs</p>

Pythium root rot disfunction microscopic signs

  • y-branching

  • oospores within the roots

<ul><li><p>y-branching</p></li><li><p>oospores within the roots</p></li></ul><p></p>
14
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<p>Take all Patch microscopic signs</p>

Take all Patch microscopic signs

(Ectotrophic) runner hyphae

<p>(Ectotrophic) runner hyphae</p>
15
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<p>Bacterial wilt microscopic signs</p>

Bacterial wilt microscopic signs

Bacteria flowing out of leaf blade

<p>Bacteria flowing out of leaf blade</p>
16
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<p>Pythium blight microscopic signs</p>

Pythium blight microscopic signs

  • y-branching

  • No cross walls

<ul><li><p>y-branching</p></li><li><p>No cross walls</p></li></ul><p></p>
17
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Brown patch occurs with high humidity and what temperature (warm or cool)

warm

18
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<p>Parts of the Microscope</p>

Parts of the Microscope

A- Eyepiece

B- Nose piece

C- Objective Lens

D- Stage

E- Light source

F- Base

G- Arm

H- Coarse focus

I- Fine focus

J- Stage control knob

K- Power switch

<p>A- Eyepiece</p><p>B- Nose piece</p><p>C- Objective Lens</p><p>D- Stage</p><p>E- Light source</p><p>F- Base</p><p>G- Arm</p><p>H- Coarse focus</p><p>I- Fine focus</p><p>J- Stage control knob</p><p>K- Power switch</p>
19
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Koch’s postulates

  1. The pathogen must be found with the diseased organism (plant)

  2. The pathogen must be isolated from a diseased organism and grown in pure culture

  3. The cultured pathogen must be used to inoculate a disease-free host and cause the same symptoms as first observed

  4. The pathogen must be re-isolated from the inoculated and compared
    - Same symptoms
    - Same pathogen