Compound Microscope

it supports the tube and connects it to the base

arm

the bottom of the microscope, used for support

base

the lens at the top that you look through, usually 10x or 15x power

eyepiece lens

connects the eyepiece to the objective lenses

tube

serves as the light source for a microscope during slide specimen visualization

illuminator or light source

a flat platform where you place your slide

stage

holds the slides in place for viewing

stage clips

part of the microscope that holds two or multiple objective lenses and helps to rotate objectives lenses and also helps to easily change power

revolving nosepiece

these are found on the nosepiece and range from 4x, 10x, 40x, and sometimes 100x powers

objective lenses

a rotating disk under the stage. this part controls the amount of light reaching the specimen. (located below microscope stage and above the condenser lenses)

diaphragm or iris

part used to collect and focus light from the illuminator on to the species. (located under diaphragm)

condenser

large knob used for focusing the image under low power

coarse adjustment knob

these allow you to move your slides

stage control

it is used to vary the light that passes through the stage opening and helps to adjust both the contrast and resolution of a specimen

brightness adjustment

turns the illuminator on and off

light switch

smaller knob used for focusing the image within the medium and high-power objective

fine adjustment knob

supports the objective lenses and ocular lenses

head

circular area on stage through which light passes

aperture

a knob or lever that adjusts the magnifier on the viewfinder to compensate for differences in vision.

diopter adjustment

why do you start with the longest objective lens before viewing?

having start with the longer lens before viewing makes it so that when you switch from 4x to 10x then 40x you won't hit the slide with the objective lens.

what is the difference between magnification and resolution?

magnification- making an image appear larger. up until the limit of resolution.

resolution- minimum distance apart that two objects can be for them to appear as two separate items.

what is the formula for magnification?

magnification = image size/actual size

do you start with the fine or coarse knob adjustment?

CORSE KNOB. it is used for initial focus by moving either the stage or the objective lenses up and down.

what is the order you should use the objective lenses in?

4x -> 10x -> 40x

do you ensure the slide touches the first objective lens?

NO. be sure there is room for the slide to move into place.

if the slide you placed on stage is not moving around when you turn the stage control what do you think you forgot to do?

adjust the stage clips properly onto the slides

what are the 3 main things you observe when looking at an onion cell?

nucleus, cell wall, cytoplasm

what are the 3 main things you observe when looking at one cheek cell

cell membrane, nucleus, cytoplasm

how much iodine solution are we putting on the onion peel?

one drop (or the lab will 💥)

what are we using to GENTLY scrape the inside lining of our cheeks?

toothpick

what is mr borg's biggest rule in the lab?

common sense :)

what drop of liquid are we putting on our slide along with our cheek cells?

methylene blue

what is the wet-mount technique

placing a fluid solution on a slide, keeping the onion cell in a solution, and then covering the onion cell and the solution with a cover slide. (makes it so that the cells are easier to stain and you can see it more visibly)

what do the focusing knobs on a microscope do?

move the stage up and down until the image is clear

how do you calculate total magnification?

ocular lens x objective lens= total magnification

how do you carry a microscope?

one hand on the arm and one hand under the base