(1) MYCVIR LEC CNS-RABIES VIRUS

Rabies Virus

A bullet-shaped, enveloped virus with linear, (-) sense ssRNA and a helical nucleocapsid

Rabies

Most lethal of all infectious dse

Furious rabies

Dumb rabies

2 forms of rabies

Furious rabies

RABIES

• Limbic/nervous system

• Headache, fever, irritability, restlessness, anxiety and muscle pain, salivation and vomiting

Dumb rabies

RABIES

• Neocortex

• Depression and paralysis followed by coma

• Death results from respiratory arrest

• Difficult to diagnose clinically

Hydrophobia

This phase of furious rabies continues until patient lapses into coma

3-8 weeks to 1 year

Incubation period of Rabies

Bite of rabid animal

Superficial abrasion of skin

Human to human via saliva

3 MOT for Rabies (BSH)

Bite of rabid animal

Most common way of transmitting rabies

Direct immunofluorescence assay

Fastest assay for rabies

Impression smears

What smear uses:

• Various areas of brain

• Biopsy of skin

• Cornea

Rapid Fluorescent Focus Inhibition Test (RFFIT)

in vitro cell culture neutralization test

measures rabies neutralizing antibody

Enzyme-linked immunosorbent assay (ELISA)

Most sensitive out of all the serological tests for rabies

CSF

spx in serological test for rabies

positive only in infected but not in vaccinized individuals

Negri bodies

Histologic exam of Rabies shows _________ bodies