Microorganisms and Pigments stains - Exam 4 pics

A

Kinyoun, Ziehl-Neelsen, or Fite stain (1)

• Mycobacteria = red

• Background = blue

A

Kinyoun, Ziehl-Neelsen, or Fite stain (2)

• Mycobacteria = red

• Background = blue

B

Kinyoun, Ziehl-Neelsen, or Fite stain (1)

• Mycobacteria = red

• Background = blue

B

C

Kinyoun, Ziehl-Neelsen, or Fite stain (1)

• Mycobacteria = red

• Background = blue

C

Troubleshooting

Blotchy staining of Fite stain.

• Did not blot/remove xylene peanut oil

Auramine-Rhodamine

• Mycobacteria = yellow

  • Background = black

Gram stain

• + is blue to purple

• - and fibrin is red

• Background is orange

Modified Giemsa Diff Quik

• H. pylori = dark blue

• Background is pink blue

Alcian Yellow-Toluidine Blue

• H. pylori = dark blue

• Mucin = yellow

• background = pale blue

PAS-Fungus (1)

• Fungi, glycogen, polysaccharides = bright red

• Background = green

Note: chromic acid will over-ox acidic glycoproteins and GaGs so X show

PAS-Fungus (3)

• Fungi, glycogen, polysaccharides = bright red

• Background = green

Note: chromic acid will over-ox acidic glycoproteins and GaGs so X show

GMS (more background, rods)

• Fungi (alive, dead), bacteria, acidic glycoproteins = black

• Background = green

Note: chromic acid will over-ox acidic glycoproteins and GaGs so X show

• Pneumocystis jirovecii require longer impregnation

GMS (circular)

• Fungi (alive, dead), bacteria, acidic glycoproteins = black

• Background = green

Note: chromic acid will over-ox acidic glycoproteins and GaGs so X show

• Pneumocystis jirovecii require longer impregnation

GMS (less background)

• Fungi (alive, dead), bacteria, acidic glycoproteins = black

• Background = green

Note: chromic acid will over-ox acidic glycoproteins and GaGs so X show

• Pneumocystis jirovecii require longer impregnation

GMS nonspecific staining

• Low Chromic acid oxidation time

• Weak oxidizer (periodic acid)

• Tissue was fixed in glytaraldehyde

GMS overstained (1)

• Overheating of silver solution

• Too much time in silver

GMS overstained (3)

• Overheating of silver solution

• Too much time in silver

GMS understaining (light green)

• Inadequate oxidation

• Low time in silver

• Silver not heated enough

GMS understaining (little bit more green)

• Inadequate oxidation

• Low time in silver

• Silver not heated enough

Warthin Starry Steiner and Diertle - GI, more yellow

• Spirochetes and bacteria = black

• Background = yellow-brown

Warthin Starry Steiner and Diertle

• Spirochetes and bacteria = black

• Background = yellow-brown

Warthin Starry Steiner and Diertle — more orange

• Spirochetes and bacteria = black

• Background = yellow-brown

Genta

• Made of Warthin/Starry/Steiner/Diertle + Alcian Blue + Hematoxylin and Eosin

• Bacteria, mucins

PB

Prussian Blue (busy)

• Ferric ions = blue

• background is red

PB

Prussian Blue (asbestos)

• Ferric ions = blue

• background is red

Turnbull blue (busy)

• Ferrous ions are blue

Turnbull blue (more background)

• Ferrous ions are blue

Schmorl Technique (no mucicarmine)

• Argentaffin substances (bile, melanin, chromaffin) and Lipofuscin = blue

Schmorl Technique (with mucicarmine)

• Argentaffin substances (bile, melanin, chromaffin) and Lipofuscin = blue

• Mucin = red-pink

Fontana-Masson (skin)

• Argentaffin substances (bile, melanin, chromaffin) and Lipofuscin = black

Fontana-Masson (lymph node)

• Argentaffin substances (bile, melanin, chromaffin) and Lipofuscin = black

Fontana-Masson (GI)

• Argentaffin substances (bile, melanin, chromaffin) and Lipofuscin = black

Troubleshooting

Fontana Masson-

Nonspecific or background staining

• Overstaining in silver solution

• Contamination

Grimelius and Churukain-Schenk (oval)

• Argentaffin and argyrophil substance = black

• Nuclei = pink

Grimelius and Churukain-Schenk (more yellow-orange)

• Argentaffin and argyrophil substance = black

• Nuclei = pink

Grimelius and Churukain-Schenk (wide)

• Argentaffin and argyrophil substance = black

• Nuclei = pink

GMS for urate crystals

• urate = black

Hall’s Bile stain Fouchet’s (pink)

• Bile/hematoidin = green

• Muscle = yellow

• Collagen = red

Hall’s Bile stain Fouchet’s (more yellow)

• Bile/hematoidin = green

• Muscle = yellow

• Collagen = red

Von Kossa (speckled)

• Calcium phosphates and argentaffin substances = brown-black

Von Kossa (vessel)

• Calcium phosphates and argentaffin substances = brown-black

Von Kossa (blood)

• Calcium phosphates and argentaffin substances = brown-black

Rhodanine Method

• Copper protein = brown-red-yellow