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Test for proteins
Biuret Test: light blue → purple
Gently shake protein solution+ biuret reagent
Test for reducing sugars - glucose
Benedict's Test - light blue → brick red
Heat in water bath
Test for non reducing sugars - sucrose
If negative result was found initially with Benedict's - add hydrochloric acid ( will hydrolyse any glycosidic bonds ) Ada hydrogen carbonate powder to neutralise had Benedict's reagent - blue → red
Test for Starch
Iodine solution- orange/brown → blue/black
Test for lipids
Ethanol- white cloudy emulsion
Lipids are hydrophobic only dissolve in organic solvents - mixt + add water -