Hematology 1b

What is the circle indicating

if large blip, could indicate nRBC or Plt clumps

What instrument? What are each arrow representing?

Left- Lymphocytes

Middle- Mononuclear cells

Right- Granulocytes

Coulter LH Series WBC 3 part differential

Coulter principle

Blood cells diluted in an electrically conductive diluent

What does this represent? The width of the peak represents?

RBC & RDW. Larger RDW has a broad curve

What does this graph represent? What would be happenin if the line was jagged?

Platelet
platelet clump

Blue- Lymphocytes

Green- Monocytes

Pink- Neutrophils

Right: Eosinophils

very very left would show nRBC or giant Plts

Blue- lymph
Green- Mono

Pink- Neutrophils

Orange- Eosinophils

Basophils are mixed behind

what are the circles represnting

immature grans

VCS flow cellnfor 5 part WBC diff principle

Cells pass thru flow cell singly by hydrodynamic focusing

Volume, conductivity, scatter

Coulter measured parameters

WBC, RBC, Plt, Hgb

Coulter derived parameters

MCV, RDW

Coulter calculated parameters

Hct, MCH, MCHC

System XE series Hemoglobin measurement

Converts ferrous Fe to ferris Fe- methemoglobin

Cyanide free method

Sysmex measured parameters

WBC, RBC, Hgb, Hct, Plt, nRBC, Diff%, Ret%, IRF

Sysmex derived parameters

MCV, RDW, MPV

Sysmex calculated parameters

MCH, MCHC, Diff#, Ret#

Cellavision

Non CBC analyzer

Automated digital cell morphology identification system

Abbott principle

Light scatter and fluorescence

MAPPS technology

Abbott forward light scatter

Cell size

Abbott 7* light scatter

Cell complexity

Abbott 90* light scatter

Nuclear lobularity

Abbott extra assasys

Fluorescent immunophenotyping methods

1. T cell assay- CD4 & CD8

2. IMMUNOplt assay- CD61

Abbott 90* depolarized light scatter

Cytoplamsic granularity

WbC histogram cell sizes

Lymphocytes 35-90fL

Mononuclear cells 90-160fL

Granulocytes 160-450 f)