Lab exam 3 amy

based on study guide

1.

Ocular lens (eyepiece)

2.

Nose piece

3.

Mechanical stage (Stage)

4.

Condenser

5.

Illumination (light source)

6.

Brightness adjustment

7.

Base

8.

Head

9.

Objective lens

10.

Frame (arm)

11.

Stage control

12.

Course adjustment

13.

Fine adjustment

14.

Light switch

15.

Diopter Adjustment

What is the function of the eyepiece on a microscope?

Contains the ocular lens.

What is the function of the nosepiece on a microscope?

Holds the objective lens.

What is the function of the objective lens on a microscope?

Increases magnification.

What is the function of the mechanical stage on a microscope?

Supports slide.

What is the function of the condenser on a microscope?

Concentrates light on specimen.

What is the function of the iris on a microscope?

Regulates amount of light.

What is the function of the Illumination on a microscope?

Projects light through condenser, specimen, and lenses.

What is the function of the arm on a microscope?

Supports microscope when carried.

What is the function of the fine adjustment on a microscope?

Moves stage slightly to sharpen image.

What is the function of the course adjustment on a microscope?

Moves stage up/down for focusing.

What is the function of the base on a microscope?

Supports microscope.

What is the function of the stage control on a microscope?

Moves stage clips & slide around the stage.

How is total magnification calculated?

Power of ocular lens/eyepiece (10) x the power of the objective lens.

Define: parfocal.

Slide stays in focus when moving from one objective to the next if it was properly focused.

Define: resolution.

Ability to distinguish between two close objects, minimum of 0.2 micrometers with these scopes. Decimal. (the shortest distance between two separate points on a specimen that allows them to be clearly distinguished as separate entities.)

Define: depth of field.

Thickness of the plane that is clearly in focus.

Define: working distance.

How far the bottom of the objective lens is from the specimen. Decreases as you increase power of objective.

Define: field.

Area you see through the microscope. Decreases as you increase power of objective.

Identify:

1. Nucleus

2. Mitochondria

3. Golgi apparatus

Identify:

1. Nucleus

2. Mitochondria

3. Golgi apparatus

4. Rough ER

5. Smooth ER

6. Lysosome

7. Centrioles

Identify the different stages.

Label: layers of the skin

Label: structures of the skin

Label: layers of epidermis

Identify:

1. epidermis

2. dermis

3. hypodermis

Layers of the epidermis

Identify:

1. Meissner’s corpuscle

2. Pacinian corpuscle

3. Arrector pili

4. Hair

5. Sebaceous gland

6. Eccrine sweat gland

7. Apocrine sweat gland

8. Dermal papillae

Identify:

1. Polar microtubules

2. Kinetochore microtubules

Identify: nuclear envelope

Identify: chromosomes

Identify: mitotic spindle