Lab 2: Microscopy and Hemacytometer Cell Count

Each living cell is bound by

plasma membrane

The cells of plants, fungi, and bacteria have an additional _______ surrounding the plasma membrane

cell wall

The eukaryotic cell contains several subcellular structures called ______, each one performing a specific task to maintain the structure and upkeep of the cell

organelles

Compound microscope make use of two glass lenses, __________ lenses

objective and ocular

The ________ lens forms a magnified intermediate image of the specimen in the tube, this image is illuminated from below the lamp. The image is further magnified by the _______ lens.

Objective, Ocular

The total magnification perceived is

the product of the magnifications of the objective and ocular lens

The resolution of a microscope is

he smallest separation between two viewed elements that allows the image to still be seen as two separate elements

What are the two different techniques you will use to view microorganisms?

Wet mount technique and Staining Techniques

Wet Mount Technique

Allows you to see organism’s true color and shape. Organism can also be difficult to see due to movement or translucency

Staining Techniques

Using one dye and staining everything the color of the dye. Can be used to see the organism and visuals differences between organisms. Unfortunately, the organisms are killed by the staining process and the staining masks an organism’s true colors and shape. It also takes more time and supplies

Microscope Parts

Hemocytometer

Thick glass microscope slide traditionally used to count the number of cells in a given volume of liquid

Number of cells per cubic millimeter

n = per square millimeter x dilution (if used) X 10

Number of cells per milliliter

n = cells counted per square millimeter X dilution X 10,000