Topic 3: Prokaryotic Cell

organelle

membrane-bound or free-floating structures with critical functionalities within the cytoplasm

prokaryotic cell

most basic cellular form with no membrane bound organelles and no true nucleus

bacteria

0.001-0.002mm in diameter, heterotrophic

cyanobacteria

green chloroplast containing, photosynthetic

cocci

round bacteria

bacilli

rod-shaped bacteria

spirilla

spiral-shaped bacteria

pure cultures

cultures containing a single isolated species of bacteria

agar

complex carbohydrate - liquid growth medium (broth) can be solid with agar - culture media is necessary for bacterial growth because of containing nutrientsused to solidify media for culturing bacteria.

subculturing

forms pure cultures - bacteria are transferred from one medium plate to another - streak plate, allowing discrete colonies - spread plate allows even lawn growth

procedure for streak plate

x

procedure for spread plate

x