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Domain
The highest level in biological classification. There are three domains: Bacteria, Archaea, Eukarya
Eukaryote
Organism composed of one or more eukaryotic cells; members of the domain eukarya are eukaryotes
Eukaryotic cell
cell type characterized by a membrane-bound nucleus
Prion
An acellular infectious agent consisting only of protein
Prokaryotic cell
Single-celled organism consisting of a prokaryotic cell; members of the domain bacteria, archaea are prokaryotes
Viroid
An acellular infectious agent consisting only of RNA
Capsule
a distinct, thick gelatinous material that surrounds some microorganisms
Chemotaxis
Movement of a cell towards or away from a certain chemical in the environment
Cytoplasmic membrane
A phospholipid bilayer embedded with proteins that surrounds the cytoplasm and defines the boundary of the cell
Endospore
An extraordinarily resistant dormant cell produced by some types of bacteria
Flagellum
A type of structure used for cell movement
Gram-negative bacteria
Bacteria that have a cell wall characterized by a thin layer of peptidoglycan surrounded by an outer membrane; when Gram stained, these cells are pink
Gram-positive bacteria
Bacteria that have a cell wall characterized by a thick layer of peptidoglycan; when Gram stained, these cells are purple
Lipopoly saccharide
Molecules that make up the outer layer of the outer membrane of Gram-negative bacteria
Peptidoglycan
A molecule that provides strength to the cell wall; it is found only in bacteria
Periplasm
The gel-like material that fills the region between the cytoplasmic membrane and the outer membrane of Gram-negative bacteria
Pili
Cell surface structures that allow cells to adhere to certain surfaces; some types are involved in a mechanism of DNA transfer
Plasmid
Extrachromosomal DNA molecule that replicates independently of the chromosome
Ribosome
Structures involved in protein synthesis
Transport system
mechanisms used to transport nutrients and other small molecules across the cytoplasmic membrane
Biofilm
polymer-encased community of microorganisms
chemically defined medium
a culture medium in which the exact chemicals; generally used only for specific experiments when nutrients must by precisely controlled
Complex medium
A culture medium with an ingredient that certain microorganisms proteins digests, extracts, or other ingredients that vary in their chemical composition
Differential medium
A culture medium with an ingredient that certain microorganisms change in a recognizable way; used to differentiate microbes based on their metabolic traits
Exponential (log) Phase
Stage in the growth curve during which cells divide at a constant rate; generation time is measured during this period of active multiplication
Focultative Anaerobes
Organisms that grow best if oxygen is present, but can also grow without oxygen
Generation Time
Time it takes for the number of cells in a population to double; doubling time
Obligate Aerobes
Organisms that have an absolute requirement for o2. They use aerobic respiration (micrococcus luteus)
Obligate Anaerobes
organisms that cannot multiply if oxygen is present
Plate count
Measures the number of viable cells in a sample by taking advantage of the fact that an isolated microbial cell on a nutrient agar plate will give rise to one colony
Pure culture
Population of organisms descended from a single cell
Selective medium
A culture medium with an ingredient that inhibits the growth of microorganisms making it easier to isolate the one being sought
Antiseptic
A disinfectant nontoxic enough to be used on skin
Aseptic technique
Procedures that help prevent the accidental introduction of unwanted microbes
Bactericide
Substance that kills bacteria
Bacteriostatic
Prevents the growth of, but does not kill bacteria
Disinfectant
A chemical that destroys many, but not all microbes
Pasteurization
Brief heating to reduce number of spoilage orgs, destroy pathogens, or disease causing microbes
Preservation
Inhibition of microbial growth to delay spoilage
Sterile
Free of all viable microbes including endospores and viruses
Sterilization
The destruction or removal of all microbes through physical or chemical means