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AQA A-LEVEL BIOLOGY PAPER 2 TOPIC 5
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What is photosynthesis?
A reaction in which light energy is used to produce glucose in plants.
What are the required compounds of photosynthesis?
carbon dioxide and water
What are the products of photsynthesis?
glucose and oxygen
Where does photosynthesis take place?
The chloroplasts
How many stages of photosynthesis are there?
2
What are the 2 stages of photosynthesis?
Light dependent reaction
Light independent reaction
Name 3 factors that may affect the rate of photosynthesis.
Carbon dioxide concentration, light intensity, temperature
What is the Light Dependent Reaction?
the first stage of photosynthesis
Where does the LDR take place?
The thylakoid membranes or the grana
What happens overall in the LDR?
Light energy and water are used to create ATP and reduced NADP, which are needed for the light independent reaction.
List the chemicals required for the LDR.
NADP
ADP
Pi
Water
What are the 4 key stages of the LDR?
Photoionisation of Chloropyll
Photolysis
Chemiosmosis
Production of ATP and reduced NADP
Describe the process of the photoionisation of chlorophyll during the LDR.
chlorophyll molecules inside the PSII absorb light
the light excites the electrons in the chlorophyll.
Electrons are lost, and the chlorophyll becomes positively charged
Some of the energy from the released electrons is used to make ATP and reduced NADP in chemiosmosis.
Describe the process of the photolysis of water in the LDR.
light energy is absorbed by the chlorophyll, splitting water into Oxygen, protons and electrons.
Describe the process of chemiosmosis during the LDR.
The electrons that were excited by the light energy and left the chlorophyll move along an electron transfer chain, releasing energy.
Some of this energy is used to pump the protons across the chloroplast membrane.
An electrochemical gradient of protons is established; the protons pass through the enzyme ATP synthase, which results in the production of ATP.
The protons combine with the co-enzyme NADP to become reduced NADP.
What type of reaction is the LIR of photosynthesis?
A cycle
What is the term used to describe the cycle that takes place during the LIR?
The calvin cycle
Where does the calvin cycle take place?
the stroma
What enzyme does the stroma of the chloroplast contain that is used in the calvin cycle?
RuBisCo
What is the purpose of RuBisCo in the Calvin Cycle?
It catalyses the reaction
Why is the Calvin Cycle temperature sensitive?
Due to the presence of the enzyme RuBisCo in the stroma of the chloroplasts.
What is hydrolysed to provide energy for the calvin cycle?
ATP
Describe the process of the Calvin Cycle.
Carbon dioxide reacts with Ribulose Biphosphate RuBP to form 2 molecules of glycerate-3-phosphate (GP). This is catalysed by rubisco.
GP is then reduced to Triose Phosphate (TP) using energy from ATP and by accepting an H from reduced NADP.
Some carbon atoms from TP leave the cycle to be converted into organic substances such as glucose.
The rest of the carbon from TP is used to regenerate RuBP with the energy from ATP.
What happens to the carbon atoms from TP that leave the cycle?
They are converted into organic substances such as glucose
What does respiration produce?
ATP
What are the 4 key stages of aerobic respiration
Glycolysis
Link Reaction
Krebs Cycle
Oxidative Phosphorylation
Where does glycolysis occur? (Respiration)
The cytoplasm
Where does the link reaction occur? (Respiration)
mitochondrial matrix
Where does the krebs cycle occur?
The mitochondrial matrix
Where does oxidative phosphorylation occur?
Mitochondrial cristae
Is glycolysis an aerobic or anaerobic process?
anaerobic
Describe the process of glycolysis in respiration.
Phosphorylation of glucose to glucose phosphate using ATP
The production of two triose phosphate (3C) by the splitting of glucose phosphate
Oxidation of triose phosphate to pyruvate
There is a net gain of ATP, an NAD is reduced
What are the products of glycolysis during respiration?
2X Pyruvate
Net gain of 2 ATP
2X NADH
Describe the process of the link reaction in respiration?
The pyruvate made in glycolysis is oxidised to acetate
NAD picks up the hydrogen and becomes reduced NAD
Acetate combines with coenzyme A to produce acetylcoenzyme A
How many times does the link reaction occur for every glucose molecule?
twice
What are the products of the link reaction during respiration?
2X Acetylcoenzyme A,
2X CO2 released,
2 reduced NAD.
Describe the process of the krebs cycle.
Acetyl CoA combines with a 4 carbon molecule, releasing coenzyme A and producing a 6 carbon compound.
The 6 carbon compound is decarboxylated into a 5 carbon compound.
A hydrogen is removed from NAD to produce reduced NAD
The 5 carbon molecule is now converted into a 4 carbon molecule.
2X NAD are reduced to reduced NAD, 1 ATP is produced and 1 FAD is reduced into reduced FAD.
What are the products per cycle of the krebs cycle?
3X Reduced NAD
1X Reduced FAD
1X ATP
2X CO2
What are the products per glucose molecule for the krebs cycle?
6X reduced NAD
2X reduced FAD
2X ATP
1X CO2