Lab Practical Flashcards

What is the term for procedures used to prevent contamination during laboratory work?

Aseptic technique

How is the total magnification of a specimen being viewed under a microscope calculated?

$$\text{Ocular magnification} \times \text{objective magnification}$$

Which specific objective lens provides $100\times$ magnification and requires a specialized oil to function correctly?

Oil immersion objective

Which part of the microscope is responsible for focusing light directly onto the specimen?

Condenser

What is the term for the ability of a microscope to keep an object nearly in focus when changing from one objective lens to another?

Parfocal

What is the purpose of a Bunsen burner in a microbiology lab?

Flame used for sterilization

What process is used to attach bacteria to a slide before the staining procedure begins?

Heat fix

What term defines a cell that lacks a membrane-bound nucleus?

Prokaryote

Into what shape is a Vibrio bacterium classified?

Comma-shaped

What prefix is used to describe a bacterial arrangement of cells in chains?

Strepto-

Which flagellar arrangement is characterized by having flagella distributed all over the surface of the cell?

Peritrichous

What is the role of iodine in the Gram stain procedure?

Mordant

After a successful Gram stain, what color do Gram-negative bacteria appear under the microscope?

Pink

Which component of the cell wall is responsible for retaining the crystal violet stain in Gram-positive bacteria?

Peptidoglycan

What type of stain is used specifically to detect bacterial capsules?

Capsule stain

In colony morphology, what term describes a colony with a raised center when viewed from the side?

Umbonate

What is the primary purpose of performing a streak plate technique?

Isolates individual colonies

In an experimental design, which variable is the one that is changed or manipulated by the experimenter?

Independent variable

Through what structures does a Paramecium caudatum achieve movement?

Cilia

What are the thread-like filaments that constitute the body of a fungus?

Hyphae

What is the name of the asexual spores formed on conidiophores in molds like Aspergillus?

Conidia

What is the term for an individual segment of a tapeworm?

Proglottid

How is the organism Escherichia coli characterized in terms of Gram reaction and shape?

Gram-negative rod

Which organism is a Gram-positive rod known for its ability to form endospores?

Bacillus subtilis

What is the name of the most specific taxonomic rank in the biological classification system?

Species