Lab 2 (Scientific Method + Microscope)

steps to the scientific method

observation, hypothesis, experimentation, data collection and analysis, conclusiom

what happens during the observation step

scientists form questions relating to the observations and find independent and dependent variables

what is a hypothesis

tentative answer to a well formed question, if then statement,

independent variable

treatment or condition being manipulated or changed or being tested

dependent variable

condition being measured

control variable

factors that are kept the same throughout the experiment

how do scientists organize data

tables or figures to see trends

what is a line graph used to measure

relationship between 2+ quantitative variables

bar graph used to show…

relationship between quantitative and qualitative data (categorical)

what should a conclusion include

major findings, whether hypothesis was true, 

most commonly used microscope

compound light microscope

compound light microscopes are…

binocular

magnification

size of an image viewed bigger than the actual size

ocular objective

10x

scanning power objective lens

4x

low power objective lens

10x

high-dry power objective lense

40x

oil immersion objective lens

100x

how to use oil immersion lense

drop oil into the slide so that it touches the slides and the lens

parfocal

when a specimens keeps focus even when changing lens

parcentral

when the specimen stays centered even when changing objectives

illuminator

light source under the base that gives the light needed (NOT UNDER STAGE)

aperture

hole on stage to let light in

slide position adjustment knob

move the slide on the stage to center field of view (knobs near the state)

condenser

disk under the stage and controlls light coming from illuminator

iris diaphragm

ON THE CONDENSER

resolution

able to see fine detail

depth of field

how thick a layer of  aspecimin is in focus

working distance

howw far the lens is from the specimin

staining

enhance the contrast of the image

as magnification increases,

working distance decreases

if slide is moved to the left, the position in view moves…

to the right