BIOCH 200 - Reactions of Glycolysis and the Citric Acid Cycle

6, 7, 10

Energy capture steps of glycolysis

1, 3

Energy investment steps of glycolysis

Hexokinase, 1 ATP

Reaction 1 of Glycolysis:

Glucose → Glucose-6-phosphate

Enzyme: ____________

Molecules used: ____________

Phosphofructokinase-1 (PFK-1), 1 ATP

Reaction 3 of Glycolysis:

Fructose 6-phosphate → Fructose 1,6-bisphosphate

Enzyme: ____________

Molecules used: ____________

Commitment step

Lysis, Isomerization

Reaction 4 and 5 of Glycolysis:

Fructose 1,6-bisphosphate ⇌ Dihydroxyacetone phosphate + Glyceraldehyde 3 - phosphate (GAP) - _________

Dihydroxyacetone phosphate ⇌ Glyceraldehyde 3 - phosphate (GAP) - _________

Energy Investment Stage - 2 ATP

Stage 1 of Glycolysis

Reactions 1-4 of Glycolysis

Glucose → 2 GAP

2 ATP

Net ATP investment in glycolysis

4 ATP

ATP generated in glycolysis

2 ATP

Net ATP generated by glycolysis

Glyceraldehyde-3-phosphate dehydrogenase (GADPH), 2 NADH, oxidation and phosphorylation

Step 6 of Glycolysis

Glyceraldehyde 3-phosphate (GAP) ⇌ 1,3-bisphosphate (1,3-BPG)

Enzyme: ____________

Molecules generated: ____________

Reaction description: _____________

2 ATP, substrate level phosphorylation

Step 7 of Glycolysis

1,3-bisphosphate (1,3-BPG) ⇌ 3-phosphoglycerate

Molecules generated: ____________

Reaction description: _____________

Isomerization

Step 8 of Glycolysis

3-phosphoglycerate ⇌ 2-phosphoglycerate

Reaction description: _____________

2 H2O, dehydration

Step 9 of Glycolysis

2-phosphoglycerate ⇌ Phosphoenolpyruvate (PEP)

Molecules generated: ____________

Reaction description: _____________

Pyruvate kinase, 2 ATP, substrate level phosphorylation

Step 10 of Glycolysis

Phosphoenolpyruvate (PEP) ⇌ Pyruvate

Enzyme: __________

Molecules generated: ____________

Reaction description: _____________

2 pyruvate, 2 NADH, 2 ATP, 2 H2O, 2 H+

5 net products of Glycolysis

3, 4, 5, 6, 8

Energy capture steps of the citric acid cycle

Pyruvate dehydrogenase, NADH, CO2

Step that links glycolysis to citric acid cycle

Pyruvate → Acetyl-CoA

Enzyme: __________

Molecules generated: ____________

Citrate synthase, H2O, CoA + H+

Step 1 of Citric Acid Cycle

Acetyl-CoA → Citrate

Enzyme: __________

Molecules generated: ____________

Isocitrate dehydrogenase, NADH, CO2

Step 3 of Citric Acid Cycle

Isocitrate → a-ketoglutarate

Enzyme: __________

Molecules generated: ____________

a-ketoglutarate dehydrogenase, NADH, CO2

Step 4 of Citric Acid Cycle

a-ketoglutarate → Succinyl-CoA

Enzyme: __________

Molecules generated: ____________

GTP, CoA, substrate level phosphorylation

Step 5 of Citric Acid Cycle

Succinyl-CoA ⇌ Succinate

Molecules generated: ____________

Reaction: _________

Succinate dehydrogenase (complex II), FADH2

Step 6 of Citric Acid Cycle

Succinate → Fumarate

Enzyme: __________

Molecules generated: ____________

H2O

Step 7 of Citric Acid Cycle

Fumarate ⇌ Malate

Molecules used: ____________

NADH

Step 8 of Citric Acid Cycle

Malate ⇌ Oxaloacetate

Molecules generated: ____________

3 NADH (7.5 ATP), FADH2, GTP

Products of 1 Acetyl-CoA from CAC