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Five Kingdom System
A classification system proposing that all organisms belong to one of five kingdoms based on phenotypic properties.
Eukaryota
Organisms with complex cells that contain a nucleus, evolved from prokaryotic cells according to the Five Kingdom System.
Prokaryote
Organisms whose cells lack a nucleus, including bacteria and archaea.
rRNA
Ribosomal RNA, used in the molecular techniques to determine evolutionary relationships in the Three Domain System.
Molecular Phylogeny
The study of evolutionary relationships based on molecular data, such as the sequence of nucleotides in rRNA.
Domains
The highest taxonomic rank that groups all living cells into three categories: Archaea, Bacteria, and Eukarya.
Bergey's Manual
A reference book that classifies prokaryotes into two domains, Archaea and Bacteria, based on molecular phylogeny.
Differential Staining
A method used for classifying microorganisms based on their cell wall structure, such as Gram staining.
PCR (Polymerase Chain Reaction)
A DNA technology used to amplify specific DNA sequences for identification of microorganisms.
Serology
A molecular identification method that measures antibodies or cytokine production against a microbial antigen.