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what happens first when you start the elisa test
first the anti gen is coated onto the test plate
what happens second
the primary antibody is added which is from patient sample
what happens third
the secondary antibody (has anti human and added enzyme)
what is the result
a color change
if a patient doesn’t have then primary anti body will a color change happen
no
what is direct elisa when it comes to antigen pathogen
patient sample
what is direct elisa when it comes to antibody
produced in lab
what is indirect elisa when it comes antigen
produced in lab
what is indirect elisa when it comes to antibody
patient sample
Is the ELISA test a "direct"
or an "indirect"
test?
indirect
what does indirect mean
An indirect ELISA detects antibodies in a patient’s sample rather than the antigen itself. The color change means the patient has antibodies, showing they’ve been exposed to the antigen
2 limitations
chemical interfere or not enough anti body the second one is the anti body unspecifically bind to the antigen