quiz5 elisa

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14 Terms

1
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what happens first when you start the elisa test

first the anti gen is coated onto the test plate

2
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what happens second

the primary antibody is added which is from patient sample

3
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what happens third

the secondary antibody (has anti human and added enzyme)

4
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what is the result

a color change

5
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if a patient doesn’t have then primary anti body will a color change happen

no

6
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what is direct elisa when it comes to antigen pathogen

patient sample

7
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what is direct elisa when it comes to antibody

produced in lab

8
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what is indirect elisa when it comes antigen

produced in lab

9
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what is indirect elisa when it comes to antibody

patient sample

10
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Is the ELISA test a "direct"

or an "indirect"

test?

indirect

11
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what does indirect mean

An indirect ELISA detects antibodies in a patient’s sample rather than the antigen itself. The color change means the patient has antibodies, showing they’ve been exposed to the antigen

12
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2 limitations

chemical interfere or not enough anti body the second one is the anti body unspecifically bind to the antigen

13
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14
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