Microscopes

with resolution is a smaller or larger value better?

smaller as you can see tinier details

what is resolution?

the ability to distinguish between two or more points that are close together

explain how a microscope works

-light from source is reflected to sample

-then passes through one of the objective lens which spreads out the light rays magnifying the image

-then through the eyepiece lens to our eyes

what is an image?

the image we see when we look down at the microscope

what is an an object?

the real object or sample that you're looking at

what are two downsides to the light microscope?

- resolution is limited to 0.2um

- any details less than that will appear blurry

what is an electron microscope?

A microscope that uses electrons and electron lenses

downsides to electron microscopes

-really big,

-very expensive and hard to use,

-specimens must be dead,

-must be stained with heavy electron element such as gold or a heavy metal

- image isn’t in colour

advantages to electron microscopes

- can be used to study sub-cellular structures,

- higher magnification

- better resolution than light microscopes

- they use electrons instead of light

what are the units of conversion for microscopy?

1m x 10 --> 1cm x 10 --> 1mm x 1000 --> 1um x 1000 --> 1nm

image size =

actual size x magnification

what are two downsides to the light microscope?

-resolution is limited to 0.2um

-any details less than that will appear blurry