Histophysiology (Exam 1)
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35 Terms
brightfield microscopy
stained, fixed cells with a bright background.
darkfield
live, non-stained specimens, dark BG
phase contrast
live, non-stained, thickness.
fluorescent
labeled with fluorescence (fluorophore/fluorochrome). useful for pathogens.
TEM
detects electrons transmitted to specimen. ultra-fine details through sample.
SEM
detects electrons transmitted to specimen. 3D image of the surface.
virtual
scanned and digitalized, used for teaching.
magnification
ratio of image vs object size
resolution
resolving power, distinguishable 2 objects
Hematoxilin
stains purple (basophilic)
eosin
stains red (acidophilic)
maison trichrome stain
fibrocollagenous tissue. red=protein, blue= collagen
periodic acid schiff
detection of yeast, glycogen, Basement membrane
simple columnar
gall bladder
simple cuboidal
thyroid and parathyroid
stratified squamous non-keratinized
esophagus
stratified squamous keratinized
cow teat
stratified columnar
cow teat
pseudostratified columnar (ciliated)
bronchus, secretion and propulsement of mucus. hass goblet cells that produced mucin.
simple squamous
lungs, diffusion
transitional
ureter, uretra and bladder
mesenchymal stem cells
mucous CT
umbilical cord
areolar tissue and dense irregular CT
reticular CT
dense regular, nuchal ligament. has elastic fibers in between.
muiltilocular adipose tissue (brown)
unilocular adipose tissue (white)
dense regular CT
hyaline cartilage- ends of bones, articular surfaces.
type II collagen
elastic cartilage
- ear
1.Contain a large number of fine to coarse, interwoven, branching elastic fibers interspersed with collagen type II fiber bundles, embedded in a relatively small amount of the ground substance. The fibers can be seen at higher magnification. verhoef stain.
2.Such composition confers flexibility and shape retention,
type 2 collagen
fibrocartilage- meniscus, IVD, pubic symphisis.
No perichondrium cover! This is different from the other two types of cartilage.
TypeI Collagen fibers/bundlespredominate
perichondrium
compact bone
trabecullar bone