Heme 2 Lab - Quiz 1

What is the major hemoglobin in the newborn

Hemoglobin F

3 multiple choice options

What is Hemoglobin F composed of

2 alpha, 2 gamma

3 multiple choice options

Site of red cell production from conception to birth

1. Yolk sac

2. Liver

3. Spleen

4. Bone marrow

3 multiple choice options

What is an inverted diff?

More lymph's than segs

Why are reticulocyte counts important?

It assesses the erythropoietic activity of bone marrow

What are reticulocytes composed of?

RNA

3 multiple choice options

What are shift reticulocytes?

Reticulocytes that leave the BM prematurely

How long does it take for shift reticulocytes to lose their reticulum

2.5 days

Normal = 1 day

3 multiple choice options

RPI calculation

(Retic count (%) * (Pt HCT / 45)) / Maturation time

% Retic calculation

(Number of reticulocytes / 1000 RBCs) * 100

Normal reference range for reticulocyte count

0.5 to 2.5%

Decreased reticulocyte count seen in:

-Aplastic anemia

-Refractory anemia

-Bone marrow hypoplasia

Increased reticulocyte count seen in:

-Hemolytic anemias

-Iron deficiency anemias receiving iron therapy

-Thalassemia

-Sideroblastic anemia

-Acute and chronic blood loss

-Newborns

Wright stained differential of a person with a high reticulocyte count may show

-Polychromasia

-Macrocytes

The 6 stages of erythrocyte development:

1. Pronormoblast – BM

2. Basophilic normoblast - BM

3. Polychromatophilic normoblast - BM

4. Orthochromic normoblast - BM

5. Reticulocyte – BM & Circulating Blood

6. Mature RBC - BM & Circulating Blood

Corrected wbc formula

((Total WBC count * 100) / (NRBC count + 100))

When do you have to perform a corrected WBC count?

If the blood smear demonstrates more than 5 NRBCs/100 WBCs

3 multiple choice options

nRBC

Normal nRBC reference range

0-5 cells per 100 white blood cells