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Which of the following terms accurately describes the replication of DNA in vivo?
semidiscontinuous
After ONE round of DNA replication in Meselson and Stahl's classic experiment, ________.
the conservative model was conclusively proven to be an inaccurate model
DNA replication proceeds ________.
semiconservatively
If DNA replication were fully conservative, how many intact parental double helices would have been detected in Meselson and Stahl's experiment after three rounds of replication?
1
The amount of DNA that is replicated from a single origin of replication is referred to as a(n) ________.
replicon
Which DNA Polymerase III subunit is not part of the clamp loading assembly?
θ
DNA replication occurs by adding ________.
dNTPs to the 3′ end of the daughter strand
DNA polymerase III adds nucleotides ________.
to the 3′ end of the RNA primer
DNA polymerase I is thought to add nucleotides ________.
in the place of the primer RNA after it is removed
Which terms accurately reflect the nature of replication of the chromosome in E. coli?
bidirectional and fixed point of initiation
Which cluster of terms accurately reflects the nature of DNA replication in prokaryotes?
fixed point of initiation, bidirectional, semiconservative
Which of the following statements about the oriC region in E. coli is incorrect?
It is directly recognized and bound by DNA helicase.
The protein responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome is coded for by the ________ gene.
dnaA
If E. coli DNA polymerase III was able to initiate DNA synthesis opposite a single-stranded template, which molecule(s) would no longer be essential?
DNA polymerase I
The discontinuous aspect of replication of DNA in vivo is caused by ________
the 5′ to 3′ polarity restriction
During DNA replication, ________ are 1000-2000 nucleotide long strands synthesized on the ________ strand to maintain the ________ of replication.
Okazaki fragments; lagging; bidirectionality
As unwinding of the helix occurs during DNA replication, tension referred to as ________ is created ahead of the replication fork. This tension is relieved by the action of ________.
supercoiling; DNA gyrase
Which of the following statement about DNA replication in E. coli is correct?
There are two molecules of DNA polymerase III per replication fork
The protein product of the E. coli dnaE gene is a component of ________.
DNA polymerase III
The activity of ________ would be quickly undone in the absence of single-stranded binding protein.
DNA helicase
DNA replication in eukaryotes ________.
initiates at multiple origins
Chromatin is defined as ________.
DNA complexed with protein
Assembly of new nucleosomes is carried out by complexes abbreviated ________
CAF
Which eukaryotic DNA polymerase would least likely be involved in repair?
α
Structures located at the ends of eukaryotic chromosomes are called ________.
telomeres
Telomerase reads ________ as a template and synthesizes ________ as a product.
RNA; DNA
The process of making DNA from an RNA template is referred to as ________.
reverse transcription
Telomerase adds nucleotides to the ________.
3′ end of the parental strand
A molecule that possesses both a protein component and an RNA component is referred to as a ________.
ribonucleoprotein
Which of the following is not an essential step in homologous recombination?
exonuclease degradation
