PROTEIN DENATURATION BC LAB

Proteins

Building blocks of life

Amine group

Nitrogen atoms attached to 2 Hydrogen Atoms

Acid group

Carbon atom double bonded w/ Oxygen atom + Hydrogen atom

Amino acid

Carbon atom + Amine group + Acid group

20 Amino Acids

Amount of essential amino acids the body needs

8 amino acids

The amount of amino acids in essential amino acids that cannot be produce by human body

Protein Denaturation

Unfolding,rearrangement of secondary&tertiary structures of proteins, w/o breaking peptide bond

Reversible Denaturation

Mild condition of denaturation, protein can be restored

Irreversible Denaturation

Drastic conditions of denaturation, protein coagulates/precipitates from solution

Heat Disrupting Hydrogen Bonds

Test wherein there is formation of Coagulate

White coagulate

Positive result in Heat Disrupting Hydrogen Bonds Test

Albumin

Greatest amount of white coagulate in Heat Disrupting Hydrogen Bond Test

Gelatin

Colorless coagulate in Heat Disrupting Hydrogen Bond test

Casein

Least amount of white coagulate in Heat diseupting hydrogen test