Culture Media Examples

Bacteriodes

• Bacteriodes Bile Esculin (BBE)

  • Selective

  • Differentiate bile resistant from bile sensitive

Group D Streptococci and Enterococci

• Bile Esculin Agar

  • Nutrient Agar base media w/ ferric citrate

  • Differential isolation & presumptive ID of both

Vancomycin resistant enterococci

• Bile Esculin Azide agar w/ Vancomycin

  • selective differential for cultivation of __________ from clinical and surveillance specimens

Brain Heart Infusion (BHI)

High protein media for growing a wide variety of bacteria

Liquid

Salmonella typhi

• Bismuth sulfite agar

  • used for isolation

  • produces black colonies

Salmonella

• Deoxycholate Citrate

  • for isolation with Shigella

• Brilliant green agar (BGA)

  • other Salmonella

• Selenite Broth

  • enrichment for the isolation

• Tetrathionate Broth

  • enrichment for __________ & proteus

Vibrio

• Alkaline peptone water

  • Enrichment

  • Liquid

• Dieudonnes media

  • selective media

• Thiosulfate Citrate Bile Salts (TCBS)

  • Selective and Differential

  • to differentiate ______ as to sucrose or non-sucrose fermenters

• Cary Blair

  • stool pathogens

Amie’s media

Transport media of respiratory specimens, to maintain viability of the organisms from the source to the laboratory.

Hemophilus spp.

• Chocolate agar (CAP)

  • Cultivation

Francisella tularensis

• Blood cysteine dextrose agar (BGA)

  • For growing

Bordetella pertussis

• Bordet-Gengou Media

  • Isolation

• Regan Lowe

  • Enrichment & selective media

Legionella spp.

• Buffered charcoal yeast extract agar with antibiotics

  • Enrichment and selective

Staphylococcus

• Chapman Stone Agar

  • Selective media for isolation

• Mannitol salt sugar

  • solid-liquefiable

  • Selective isolation

Campylobacter spp.

• Campy blood agar

  • selective

• Campylobacter Thioglycollate Broth

  • Selective holding medium for the recovery

• Cefoperazone, vancomycin, Amphotericin (CVA)

  • Selective media for isolation

• Skirrow Agar

  • selective

Cary and Blair

Transport media

Separating Nocardia &

• Casein media

  • used for separating

  • Made from skimmed milk

  • used to test for hydrolysis

Yersinia spp.

• Cefsulodin-Irgasan-Novobiocin (CIN)

  • Selective

Aeromonas spp.

• Cefsulodin-Irgasan-Novobiocin (CIN)

  • may be useful for isolation

Pathogenic Neisseria

• Enriched media

• Chocolate agar (CAP)

  • Cultivation

• Cystine Tripticase Agar (CTA)

  • CTA with carbohydrates added is excellent for determining the fermentation reactions of the groups

• JEMBEC, Transgrow

  • transport media

Blood agar (BA)

cultivation of a Fastidious microorganisms used in determining hemolytic reactions

Clostridium spp.

• Chopped meat glucose

  • used for primary isolation of anaerobes

• Egg yolk agar

  • Used for the isolation and identification

Gram-positive cocci

• Columbia Colistin Nalidixic acid agar ( CNA)

Candida albicans

• Cornmeal agar with 1% Tween 80

  • use the stimulate production of Chlamydospores

Corynebacterium diptheria

• Cystine Tellurite Blood Agar

  • isolation

  • selective media

  • inhibitor:

    • Potassium tellurite

• Leoffler’s coagulated serum

  • Used for

C. Difficile

• Cycloserine Cefoxitin Fructose Agar (CCFA)

  • Selective media

Gram (-) enteric bacilli: lactose and non lactose fermenters

Lactose: Pink-purple

Non-Lactose: colorless

• Deoxycholate Agar

  • used to differentiate lactose fermenters from non-lactose fermenters organisms

• Eosin methylene blue

  • Solid-liquefiable

  • Isolation and differentiation of lactose fermenting and non-lactose fermenting

• Mac Conkey Agar

  • peptone base media with lactose

  • solid-liquefiable

  • Used for the isolation and differentiation of lactose and non-lactose fermenting

  • selective media:

    • gm (-) bacilli

  • inhibitor:

    • crystal violet

Gram (-) enteric bacilli: differentiate

• MRVP Broth

  • Methyl red and Vogues Proskauer tests

  • Biochemical test for identification

  • Biochemical test media.

• Triple Sugar Iron Agar (TSI)

  • Houston via chemical tests to differentiate

  • Carbohydrate fermentation test.

  • Biochemical test media.

Shigella

• Deoxycholate Citrate

  • for isolation with Salmonella

Ureaplasma & Mycoplasma

• “E” agar and Edward Hayflick agar

  • selective media for the eye isolation

Leptospira Interrogans

• Eillnghausen, McCullough Johnson and Harris media (EMJH)

  • Selective media

H. ducreyi

• Fetal bovine serum with vancomycin

  • selective media

Haemophilus influenza

• Enriched media

• Fildes Enrichment

  • Used for isolation

Gram negative broth (GN)

Selective enrichment (liquid) medium for enteric pathogens

Salmonella & Shigella spp.

• Deoxycholate Citrate

  • for isolation

• Hektoen-Enteric (HEA)

  • Differential, selective media for the isolation and identification from enteric bacilli

• (SSA)

  • Selective

• Tetrathione Broth

  • Selective

• Selenite broth

  • Enrichment

• Xylose Lysine Desoxycholate Agar

  • isolation and differentiation of _______ &______ from other eneteric bacilli

Gardnerella vaginalis

• Human Blood Bilayer tween

  • Biphasic

  • selective media for the isolation

  • also enriched media

Mycobacterium tuberculosis

• Lowenstein Jensen Media

  • used for the isolation

  • Malachite green is used to inhibit all organisms except_______

  • selective media

  • inhibitor: Malachite green

• Petragnani’s media

  • more malachite green

Lowenstein Jensen Media

Used for the isolation of Mycobacterium

Malachi green is used to inhibit all organisms except for this

Escherichia coli

• Mac Conkey Sorbitol Agar

  • modified that contains D-sorbitol

  • For the selection and differentiation in stool specimens

Listeria monocytogens

• Mc Bride Media

  • for the isolation

Borrelia species

• Modified Kelly’s medium or Barbour Stoenner Kelly

  • Selective media for the isolation

Mycobacterium tuberculosis

• Middle brook 7H10 or 7H11

  • Isolation

Neisseria gonorrhea

• New York City Agar

  • selective

gm(+) cocci and anaerobic gm(+) bacilli

• Phenylethyl Alcohol Agar (PEA)

  • selective isolation

Fungus culture

• Sabouraud Dextrose

• Maltose Sugar

Recovery of anaerobes and aerobes

• Schaedler Agar

  • non-selective media for the recovery

Simmon Citrate Agar

Green colored media used to test the organisms ability to use citrate as a source of carbon.

Streptococcus agalactiae

• Streptococcal Selective Agar

  • selective for __________ in female genital specimens

• Todd Hewitt

  • supplemented with antibiotics

  • Selective and enrichment in female genital specimens

Thioglycollate Broth

Supports growth of anaerobes, aerobes, microaerophilic, & fastidious organisms.

Neisseria gonorrhoea & Neisseria meningitidis

• Thayer Martin Agar

  • selective media

  • inhibitor:

    • antibiotics

Urea Agar

Used for Urease test to detect urease production which is ideal for identification of Proteus

Sulfide Indole Motility

Motility and Production test medium

Biochemical test to detect H2S production

Semi-solid

Mueller Hinton Agar (MHA)

Antibiotic susceptibility testing

Stuart’s

Viral Transport media

Urea Agar

urease test

LIA

Decarboxylase & Deaminase test