CULTURE MEDIA

Agar solidifies at?

40-50C

Agar melts at?

80-90C

Cooling temperature for distribution of media to plate

55-60C

Amount of media used

20-25 mL

Tubed media steps

Weigh

Dissolve

Dispense

Sterilize

Plated media steps

Weight

Dissolve

Sterilize

Dispense

In plated media, what is used as a your dissolving liquid?

Distileld or deionized water

Culture used in quality control procedures

Stock culture

Best way to obtain pure culture

Streak-plate method

Solidifying agent / agar

Liquid

0%

Solidifying agent / agar

Semi-solid

0.5-1%

Solidifying agent / agar

Solid

2-3%

Solidifying agent / agar

Biphasic

Both liquid and solid

Types of media as to use

Contains only what is needed to support bacterial growth

General purpose

Types of media as to use

To enhance bacterial growth

Enrichment media

Types of media as to use

For growing fastidious organisms

Enriched media

Types of media as to use

Carrot broth used for enrichment of?

S. agalactiae

Types of media as to use

Enriched media contains?

Blood

Types of media as to use

Organisms that can be grown on enriched media

Haemophilus

Neisseria

Types of media as to use

Used to promote growth of a particular organism and at the same time prevent growth of others

Selective media

Types of media as to use

Selective media contains?

Inhibitors

Cystine tellurite blood agar used for?

C. diphtheriae

CTBA inhibitor:

Potassium tellurite

Types of media as to use

Used to differentiate organisms that are growing together

Differential media

Types of media as to use

Examples of differential media

EMB, MAC, TCBS

Types of media as to use

Selective and differential examples

EMB, MAC

Transport media for Neisseria

JEMBEC

Transport media for respiratoy samples

Amie’s

Transport media for VTM

Stuart’s

Transport media for S. agalactiae

Todd Hewitt & Lim Broth (Modified Todd Hewitt)

Transport media for stool pathogens

Cary-Blair

Transport media for Neisseria (2)

Transgrow

Agar used to differentiate aerobic actinomycetes

Tap water agar

Involves successive dilution of organism until you have the cells at low density

Isolation streaking

Gross colony characteristics

Smooth, glistening, clay like

N. elongata

Gross colony characteristics

Buttery, creamy yellow

S. aureus

Gross colony characteristics

Brittle, crumbly, wrinkled

Nocardia spp

Gross colony characteristics

Fried egg on BAP

Mycoplasma

Gross colony characteristics

Shiny half pearls on BAP

Mercury droplets

B. pertussis

Gross colony characteristics

Green metallic sheen on BAP

P. aeruginosa

Gross colony characteristics

Green metallic sheen on EMB

E. coli

Gross colony characteristics

Serrated confluent growth on cetrimide

P. aeruginosa

Gross colony characteristics

Gram (-) swaming on SBA / Choc agar

Proteus spp.

Gross colony characteristics

Gram (+) swarming on SBA / Choc agar

C. tetani + C. septicum

Gross colony characteristics

Dome shaped young colonies in 24 hrs

S. pneumoniae

Gross colony characteristics

Coin with raised rim

Flat with penny edge

Aged colonies of S. pneumoniae

Gross colony characteristics

Gram (+) bull’s eye colonies

S. agalactiae

Gross colony characteristics

Hockey puck colonies

Movable colonies

Wagon wheel appearance

M. catarrhalis

Gross colony characteristics

Beaten egg white consistency

B. anthracis

Gross colony characteristics

Swirling projections

B. anthracis

Gross colony characteristics

Medusa head, lion head

Comet tail

B. anthracis

Gross colony characteristics

Gray to black on CTBA

C. diphtheriae

Gross colony characteristics

Black colonies with brown halo on Tinsdale

C. diphtheriae

Gross colony characteristics

Salmon pink pigmentation on BAP

Rhodococcus equi

Gross colony characteristics

Cauliflower like growth

M. tuberculosis

Gross colony characteristics

Bird’s nest on Corn meal

M. xenopi

Gross colony characteristics

Water droplet appearing colonies

M. bovis

Gross colony characteristics

Oxidase (-) bacteria that appears as Bull’s eyes on CIN

Y. enterocolitica

Gross colony characteristics

Oxidase (+) bacteria that appears as Bull’s eye on CIN

Aeromonas

Gross colony characteristics

Hammered copper colonies on BAP

Y. pestis

Gross colony characteristics

Non-fermenter that exhibits wrinkled colonies

P. stutzeri

Gross colony characteristics

Wrinkled colonies with earthy odor

B. pseudomallei

Gross colony characteristics

Sattelitism near incorporated V factor

H. influenzae

Gross colony characteristics

Molar tooth colonies

Actinomyces israelii

Gross colony characteristics

Medusa head with swarming

Clostridium septicum

Gross colony characteristics

Speckled and smooth

Bread crumb like

Greening on agar on exposure to air

F. nucleatum

Gross colony characteristics

Sweet, fetid odor

Peptostreptococcus anaerobius

Gross colony characteristics

Fried egg

Fusobacterium varium

Gross colony characteristics

Raspberry like colonies

Leptotrichia

Gross colony characteristics

Brick red flurres

Prevotoella porphyromonas

Gross colony characteristics

Anaerobes showing red fluorescence under UV

Veilonella

Gross colony characteristics

Anaerobe that can pit and corrode agar

B. urealyticus

Gross colony characteristics

Pits and corrodes agar + bleach like odor

Eikenella corrodens

Gross colony characteristics

Old sock odor on MSA

S. aureus

Inverted fir tree or inverted pine tree on gelatin media

B. anthracis

Fluoresces chartreuse on BAP

C. difficile

Horse stable or barn yard odor

C. difficile

Umbrella like growth on agar surface

L. monocytogenes

Test tube brush / pipe cleaner pattern

Erysipelothrix rhusiopathiae

Burnt chocolate / gun powder odor

Proteus spp.

Gram (-) yellow pigment that intensifies at RT

C. sakazakii

Dirty, musty odor like potatoes

Serratia odorifera

Produces prodigiosin - brick red pigment

S. rubidea

S. marcesens

Overripe grapes odor

P. aeruginosa

Corn taco / corn tortilla like odor

P. aeruginosa

Produces violacein

C. violaceum

Ammonium cyanide odor

C. violaceum

Brown-black pigmentation

Prevotella melaninogenica

Blue bacteria

Kluyvera spp.

Putrid odor

P. mirabilis

Freshly plowed field odor

Nocardia

Ground glass appearance

Legionella

Non-culturable but can be maintained using Mice foot pads or foot pads of armadillo

M. leprae

M. leprae can be kept alive using?

Mice foot pads or foot pads of armadillo

Breadcrumbs, puff balls / fluff balls

S. moniliformis

Speckled group colonies

F. nucleatum

Stalactite growth

Y. pestis

Butter scotch odor

S. anginosus

Produces pyocyanin and pyoverdin

P. aeruginosa