A monosaccharide derived from the catabolism of complex sugars and also known as the main source of energy in the body.
Glucose
The stored form of carbohydrates in animals.
Glycogen
It is where the absorbed glucose from the diet can be utilized directly by the cells to produce Adenosine Triphosphate (ATP)
Glycolysis
Where absorbed glucose can be stored in the muscles and liver as glycogen
Glycogenesis
The absorbed glucose can be released back into the circulation when levels are depleted.
Glycogenolysis
It is the main regulatory hormone of glucose.
Insulin
It aims to assess the overall carbohydrate homeostasis of an individual.
Glucose Measurement
Give the 6 diseases for Glucose Measurement
(Format: ccc, bbb, ddd, eee)
Hypoglycemia, hyperglycemia, glycogen storage diseases, G6PD, galactosemia, fructosuria
Greater than 500mg/dL indicate as
[ Format: blahblah (nyenye) ]
critical value (organ failure)
Greater than normal indicate as
hyperglycemia
70 - 100mg/dL indicate as
normal range of glucose
50 - 55mg/dL indicate as? Is it reversible or irreversible?
(Format: xx, yy)
hypoglycemia, reversible
<35mg/dL indicate as
[ Format: blahblah (nyenye) ]
critical value (unconscious)
<30g/dL indicate as? is it reversible or irreversible?
(Format: xx, yy)
Brain damage, irreversible
What are the 3 routinely specimen for chemical and enzymatic method for Glucose?
(Format: blahblah, blehbleh, blihblih)
Serum, plasma, whole blood
It is the most routinely used and considered as the screening method of glucose.
Glucose oxidase method
It is the reference method for glucose.
Hexokinase method
Fasting requirement for glucose according to CLSI.
8 - 12 hours
How many carbohydrate intake 3 days before blood collection
150g/day
It is an antiglycolytic tube used for glucose measurement.
(Format: blahblah - blehbleh blih blih)
Gray top tube - Sodium Fluoride
Give the serum tubes used for glucose measurement.
[ Format: Hatdog, Cheese (mayo/ketchup), Buns ]
Gold, Red (glass/plastic), Orange
Required Temperature for specimen transport in glucose measurement?
2 - 8C with cold packs
What are the rejection criteria for glucose measurement?
(Format: sinigang na baboy, tinolang manok, hotdog with tinapay, adobong manok, beef kaldereta, beef pares)
Collection tube used is wrong, improperly labeled specimen, quantity not sufficient, overfast specimen, no requisition form, hemolyzed sample
It is an enzymatic indicator test reaction that is quantified by the formation of a pink quinonimine dye.
Trinder reaction
Identify the reaction:
Glucose oxidase
Identify the reaction:
Peroxidase
End color product for glucose.
Pink
Dye used in glucose by catalyzing peroxidase, which reacts with phenol and 4-aminoantipyrine.
Pink Quinonimine Dye
It is where glucose is determined after the enzymatic oxidation.
Glucose oxidase
Volume added for reagent.
1000uL
Volume added for distilled water.
10uL
Volume added for sample.
10uL
Incubation period for glucose.
(KAHIT ANO SA DALAWA. PAG MALI KAYO HERE, CONSIDERED AS TAMA PA RIN)
10 minutes at 15 - 25C in water bath
5 minutes at 37C
It should be stable for?
60 minutes
Wavelength for glucose.
500nm
Reference range for glucose in conventional unit
70 - 100mg/dL
Reference range for glucose in SI unit
3.9 - <5.6mmol/L
Conversion factor for glucose to convert it into SI unit
0.0555
What is the conventional unit for glucose?
mg/dL
What is the SI unit for glucose?
mmol/L
What is multiplication factor for glucose?
100mg/dL
What is the interference for glucose measurement?
Hemolyzed sample
Enumerate the 3Ps of Diabetes: (in any order)
[ Format: pppp, ssss, qqqq ]
TAMA KA PA RIN BASTA MA ENUMERATE MO YUNG TATLO
Polydipsia, polyuria, polyphagia
Excessive Thirst.
Polydipsia
Excessive food intake.
Polyphagia
Excessive urination
Polyuria