L4 hemoglobin as a model for protein regulation

Cells need a continuous supply of oxygen to produce energy. Oxygen is__ in aqueous environments like blood; therefore diffusion alone__the oxygen demands of the body. Does the body need specialized oxygen binding proteins?

Sparingly soluble, cannot meet, yes

why are hb and mb necessary if they bind the same molecule? (simple answer)

body needs to be efficient at storing + release, and transport

5 gen characteristics of hb

4 poly pep chains, 4 heme groups, 4 O2 groups, O2 transport, in RBC

5 gen characteristics of mb

1 poly pep chains, 1 heme group, 1 O2 groups, O2 storage, muscles

Hb demonstrates cooperativity, which is ___

binding oxygen to one of unit influence the others

Why is hemoglobin optimized for delivery and Mb for retention?

transport proteins must be willing to let oxygen go and storage protein should hold oxygen very tightly

Essentially one sub unit equals__binding behavior and for some units equals__ binding behavior

simple; co-op

Hb has _, Mb has ___ curve

sigmoidal, hyperbolic

Co-op binding facilitates __in the lungs and__in the tissues

oxygen loading; oxygen unloading

Hemoglobin exists in __. The__has a higher affinity for oxygen. The__has a lower affinity for oxygen oxygen binding shifts hemoglobin__the R state. Factors that stabilize the T state promote__.

two interconvertible conformational states, R state, T state, toward, oxygen release

Successful tissue maintenance and repair depends on adequate__

Oxygen delivery

in certain disease e.g. sickle cell, change in 1 AA → change __ function → change __ → pt management considerations

Hb, O2 delivery