BETA HEMOLYTIC STREPTOCOCCI

S. pyogenes & S. agalactiae

BETA HEMOLYTIC STREPTOCOCCI are _____ and ______

LANCEFIELD

Beta Hemolytic Streptococci are classified under _________ CLASSIFICATION

LANCEFIELD CLASSIFICATION

most significant in identifying and classifying Beta Hemolytic Streptococci; based on the extraction of C carbohydrate from the Streptococcal cell wall

throat

Group A – Streptococcus pyogenes is Regarded as major ______ pathogen

S. pyogenes

Can cause: Pharyngitis

Scarlet fever

pharyngitis with rashes

Necrotizing colitis

rapidly progressing skin infection from s. pyogenes

Erysipelas

skin infection from s. pyogenes

Post Sequela

acute glomerular nephritis and rheumatic fever from s. pyogenes

Bacitracin disk test and PYR test

Identification test for s. pyogenes

sensitive

s. pyogenes result in bacitracin disk test

positive

s. pyogenes result in PYR test

TAXO A

Other name for Bacitracin is ________

PYR Test

identification test for s.pyogenes and enterococcus

BACITRACIN DISK TEST

identification test for s.pyogenes and to differentiate staph wihich is resistant from micrococcus which is sensitive

neonatal meningitis

Group B – Streptococcus agalactiae # 1 cause of _________

capsule

Virulence Factor of Group B – Streptococcus agalactiae is _________

swabs

Detection of GBS in pregnant women is accomplished by collecting vaginal and rectal material with ______

todd hewitt or lim broth

for detection of GBS samples should be inoculated into selective broth, such as ______ or ______

todd hewitt

Screening broth for Group B – Streptococcus agalactiae _____________________ (selective, differential)

Hippurate hydrolysis and CAMP test

Identification Tests of s. agalactiae that gives positive result

BACITRAACIN DISK TEST

Identification Tests of s. agalactiae that resuylts in resistance

HIPPURATE HYDROLYSIS TEST

The positive result is due to the organism’s ability to produce The enzyme HIPPURATE HYDROLASE / HIPPURICASE

ferric chloride

HIPPURATE HYDROLYSIS TEST to detect benzoic acid

Ninhydrin reagent

HIPPURATE HYDROLYSIS TEST to detect glycine which is faster

Christie Atkins Munch Peterson

CAMP TEST

BAP

CAMP test Required media ______

s. aureus

CAMP test known organism ___________

perpendicular streak

CAMP test Manner of streaking _______

positive

which result of CAMP test enhanced hemolysis as shown by arrow head zone of beta hemolysis

negative

which result of CAMP test has NO enhanced hemolysis